@article{53040134bb3d46eb8728dbac68ade05f,
title = "A Far-Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug-Susceptibility Testing",
abstract = "Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green-emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose-based fluorogenic probe featuring a molecular rotor turn-on fluorophore with bright far-red emission (RMR-Tre). RMR-Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Aided by the red-shifted molecular rotor fluorophore, RMR-Tre exhibited up to a 100-fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR-Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool.",
keywords = "Fluorescence, Molecular Rotor, Mycobacteria, Trehalose, Tuberculosis",
author = "Nicholas Banahene and Gepford, {Dana M.} and Biegas, {Kyle J.} and Swanson, {Daniel H.} and Hsu, {Yen Pang} and Murphy, {Brennan A.} and Taylor, {Zachary E.} and Irene Lepori and Siegrist, {M. Sloan} and Andr{\'e}s Obreg{\'o}n-Henao and {Van Nieuwenhze}, {Michael S.} and Swarts, {Benjamin M.}",
note = "Funding Information: This work was supported by NSF CAREER Award 1654408 (B.M.S.), Camille and Henry Dreyfus Foundation Henry Dreyfus Teacher-Scholar Award TH-17-034 (B.M.S.), NIH R35 Award GM136365 (M.S.V.), NIH DP2 Award AI138238 (M.S.S.). NMR instrumentation was supported by NSF MRI Award 2117338 (B.M.S.). We thank Drs. Mallary Greenlee-Wacker, Mireille Kamariza, Bavesh Kana, and Ruby Dewy for helpful discussions, the Indiana University NMR and MS facilities, and the Colorado State University Flow Cytometry Core (RRID: SCR_022000). Funding Information: This work was supported by NSF CAREER Award 1654408 (B.M.S.), Camille and Henry Dreyfus Foundation Henry Dreyfus Teacher‐Scholar Award TH‐17‐034 (B.M.S.), NIH R35 Award GM136365 (M.S.V.), NIH DP2 Award AI138238 (M.S.S.). NMR instrumentation was supported by NSF MRI Award 2117338 (B.M.S.). We thank Drs. Mallary Greenlee‐Wacker, Mireille Kamariza, Bavesh Kana, and Ruby Dewy for helpful discussions, the Indiana University NMR and MS facilities, and the Colorado State University Flow Cytometry Core (RRID: SCR_022000). Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",
year = "2023",
month = jan,
day = "9",
doi = "10.1002/anie.202213563",
language = "English",
volume = "62",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
number = "2",
}
