TY - JOUR
T1 - A Far-Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug-Susceptibility Testing.
AU - Banahene, N
AU - Swarts, Benjamin
N1 - Funding Information:
This work was supported by NSF CAREER Award 1654408 (B.M.S.), Camille and Henry Dreyfus Foundation Henry Dreyfus Teacher-Scholar Award TH-17-034 (B.M.S.), NIH R35 Award GM136365 (M.S.V.), NIH DP2 Award AI138238 (M.S.S.). NMR instrumentation was supported by NSF MRI Award 2117338 (B.M.S.). We thank Drs. Mallary Greenlee-Wacker, Mireille Kamariza, Bavesh Kana, and Ruby Dewy for helpful discussions, the Indiana University NMR and MS facilities, and the Colorado State University Flow Cytometry Core (RRID: SCR_022000).
Funding Information:
This work was supported by NSF CAREER Award 1654408 (B.M.S.), Camille and Henry Dreyfus Foundation Henry Dreyfus Teacher‐Scholar Award TH‐17‐034 (B.M.S.), NIH R35 Award GM136365 (M.S.V.), NIH DP2 Award AI138238 (M.S.S.). NMR instrumentation was supported by NSF MRI Award 2117338 (B.M.S.). We thank Drs. Mallary Greenlee‐Wacker, Mireille Kamariza, Bavesh Kana, and Ruby Dewy for helpful discussions, the Indiana University NMR and MS facilities, and the Colorado State University Flow Cytometry Core (RRID: SCR_022000).
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2023/1/9
Y1 - 2023/1/9
N2 - Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green-emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose-based fluorogenic probe featuring a molecular rotor turn-on fluorophore with bright far-red emission (RMR-Tre). RMR-Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Aided by the red-shifted molecular rotor fluorophore, RMR-Tre exhibited up to a 100-fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR-Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool.
AB - Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green-emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose-based fluorogenic probe featuring a molecular rotor turn-on fluorophore with bright far-red emission (RMR-Tre). RMR-Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no-wash, low-background fluorescence detection of live mycobacteria. Aided by the red-shifted molecular rotor fluorophore, RMR-Tre exhibited up to a 100-fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR-Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool.
M3 - Article
SN - 1433-7851
VL - 62
SP - e202213563
JO - Angewandte Chemie (International ed. in English)
JF - Angewandte Chemie (International ed. in English)
IS - 2
ER -
