A method for the sequential colorimetric determination of serum triglycerides and cholesterol

Ajit Sharma, Joseph D. Artiss, Bennie Zak

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

A simple spectrophotometric method for the sequential determination of triglycerides and cholesterol from a single serum sample was developed. In this two-stage procedure, the triglycerides and cholesterol esters are first hydrolysed to glycerol and free cholesterol respectively, with simultaneous scavenging of the liberated free fatty acids, a technique that ensures clarity of the sample. The glycerol is subsequently reacted to result in an intense red chromogen with a peak absorption maximum at 510 nm following a series of enzymic reactions. In the second stage, addition of cholesterol oxidase leads to oxidation of free cholesterol generated from the cholesterol esters in the first stage and the free cholesterol normally present in the sample, yielding in a similar fashion the identical red chromogen whose absorbance is also measured at 510 nm. Results obtained with the proposed method demonstrate good correlation with established individual procedures for triglycerides and cholesterol.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalClinical Biochemistry
Volume20
Issue number3
DOIs
StatePublished - Jun 1987

Keywords

  • analysis, sequential
  • cholesterol
  • hyperlipidemia
  • triglycerides

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