TY - JOUR
T1 - A model to assess the accuracy of detecting arboviruses in mosquito pools
AU - Vitek, Christopher J.
AU - Richards, Stephanie L.
AU - Robinson, Heather L.
AU - Smartt, Chelsea T.
PY - 2009/9
Y1 - 2009/9
N2 - Vigilant surveillance of virus prevalence in mosquitoes is essential for risk assessment and outbreak prediction. Accurate virus detection methods are essential for arbovirus surveillance. We have developed a model to estimate the probability of accurately detecting a virus-positive mosquito from pooled field collections using standard molecular techniques. We discuss several factors influencing the probability of virus detection, including the number of virions in the sample, the total sample volume, and the portion of the sample volume that is being tested. Our model determines the probability of obtaining at least 1 virion in the sample that is tested. The model also determines the optimal sample volume that is required in any test to ensure a desired probability of virus detection is achieved, and can be used to support the accuracy of current tests or to optimize existing techniques.
AB - Vigilant surveillance of virus prevalence in mosquitoes is essential for risk assessment and outbreak prediction. Accurate virus detection methods are essential for arbovirus surveillance. We have developed a model to estimate the probability of accurately detecting a virus-positive mosquito from pooled field collections using standard molecular techniques. We discuss several factors influencing the probability of virus detection, including the number of virions in the sample, the total sample volume, and the portion of the sample volume that is being tested. Our model determines the probability of obtaining at least 1 virion in the sample that is tested. The model also determines the optimal sample volume that is required in any test to ensure a desired probability of virus detection is achieved, and can be used to support the accuracy of current tests or to optimize existing techniques.
KW - Arboviruses
KW - Infection
KW - Probability of detection
KW - Surveillance
UR - http://www.scopus.com/inward/record.url?scp=73349102869&partnerID=8YFLogxK
U2 - 10.2987/09-5860.1
DO - 10.2987/09-5860.1
M3 - Article
C2 - 19852231
AN - SCOPUS:73349102869
VL - 25
SP - 374
EP - 378
JO - Journal of the American Mosquito Control Association
JF - Journal of the American Mosquito Control Association
SN - 8756-971X
IS - 3
ER -