A morphological and structural study of the larval shell from the abalone Haliotis tuberculata

E. Jardillier; M. Rousseau; A. Gendron-Badou; F. Fröhlich; D. C. Smith; M. Martin; M. N. Helléouet; S. Huchette; D. Doumenc; S. Auzoux-Bordenave

doi:10.1007/s00227-008-0966-3

A morphological and structural study of the larval shell from the abalone Haliotis tuberculata

E. Jardillier, M. Rousseau, A. Gendron-Badou, F. Fröhlich, D. C. Smith, M. Martin, M. N. Helléouet, S. Huchette, D. Doumenc, S. Auzoux-Bordenave

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Abstract

The larval shell of the marine gastropod Haliotis tuberculata was investigated by polarised light microscopy, scanning electron microscopy, Raman microspectroscopy and infra-red spectroscopy. Trochophore and veliger larval sections were used for histological examination of the growing shell and each larval stage was related to the shell development and the appearance of calcified formations. We determined the stage of initial calcification by specific staining combined with polarised light examination. The shell of 30-h-old pre-veliger larvae was found to be mineralized, confirming that calcification occurred before larval torsion. Using both infra-red and Raman spectroscopy, we showed that CaCO₃ deposition occurred at the pre-veliger stage and that the mineral phase initially deposited was essentially composed of aragonite.

Original language	English
Pages (from-to)	735-744
Number of pages	10
Journal	Marine Biology
Volume	154
Issue number	4
DOIs	https://doi.org/10.1007/s00227-008-0966-3
State	Published - Jun 2008
Externally published	Yes

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@article{2cafae91f4564e638665c28a4f311efc,

title = "A morphological and structural study of the larval shell from the abalone Haliotis tuberculata",

abstract = "The larval shell of the marine gastropod Haliotis tuberculata was investigated by polarised light microscopy, scanning electron microscopy, Raman microspectroscopy and infra-red spectroscopy. Trochophore and veliger larval sections were used for histological examination of the growing shell and each larval stage was related to the shell development and the appearance of calcified formations. We determined the stage of initial calcification by specific staining combined with polarised light examination. The shell of 30-h-old pre-veliger larvae was found to be mineralized, confirming that calcification occurred before larval torsion. Using both infra-red and Raman spectroscopy, we showed that CaCO3 deposition occurred at the pre-veliger stage and that the mineral phase initially deposited was essentially composed of aragonite.",

author = "E. Jardillier and M. Rousseau and A. Gendron-Badou and F. Fr{\"o}hlich and Smith, {D. C.} and M. Martin and Hell{\'e}ouet, {M. N.} and S. Huchette and D. Doumenc and S. Auzoux-Bordenave",

note = "Funding Information: Acknowledgments This work was supported by grants from the Centre National de la Recherche ScientiWque (CNRS, Paris, France) and the Mus{\'e}um National d{\textquoteright}Histoire Naturelle (MNHN, Paris, France). We thank G{\'e}rard Mascarel for his assistance in electron microscopy work. We also thank Professor Jean-Pierre Cuif for his helpful discussions and Dr. Andrea Bullock for her critical reading of the manuscript. The experiments complied with the current French laws.",

year = "2008",

month = jun,

doi = "10.1007/s00227-008-0966-3",

language = "English",

volume = "154",

pages = "735--744",

journal = "Marine Biology",

issn = "0025-3162",

publisher = "Marine Biology",

number = "4",

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T1 - A morphological and structural study of the larval shell from the abalone Haliotis tuberculata

AU - Jardillier, E.

AU - Rousseau, M.

AU - Gendron-Badou, A.

AU - Fröhlich, F.

AU - Smith, D. C.

AU - Martin, M.

AU - Helléouet, M. N.

AU - Huchette, S.

AU - Doumenc, D.

AU - Auzoux-Bordenave, S.

N1 - Funding Information: Acknowledgments This work was supported by grants from the Centre National de la Recherche ScientiWque (CNRS, Paris, France) and the Muséum National d’Histoire Naturelle (MNHN, Paris, France). We thank Gérard Mascarel for his assistance in electron microscopy work. We also thank Professor Jean-Pierre Cuif for his helpful discussions and Dr. Andrea Bullock for her critical reading of the manuscript. The experiments complied with the current French laws.

PY - 2008/6

Y1 - 2008/6

N2 - The larval shell of the marine gastropod Haliotis tuberculata was investigated by polarised light microscopy, scanning electron microscopy, Raman microspectroscopy and infra-red spectroscopy. Trochophore and veliger larval sections were used for histological examination of the growing shell and each larval stage was related to the shell development and the appearance of calcified formations. We determined the stage of initial calcification by specific staining combined with polarised light examination. The shell of 30-h-old pre-veliger larvae was found to be mineralized, confirming that calcification occurred before larval torsion. Using both infra-red and Raman spectroscopy, we showed that CaCO3 deposition occurred at the pre-veliger stage and that the mineral phase initially deposited was essentially composed of aragonite.

AB - The larval shell of the marine gastropod Haliotis tuberculata was investigated by polarised light microscopy, scanning electron microscopy, Raman microspectroscopy and infra-red spectroscopy. Trochophore and veliger larval sections were used for histological examination of the growing shell and each larval stage was related to the shell development and the appearance of calcified formations. We determined the stage of initial calcification by specific staining combined with polarised light examination. The shell of 30-h-old pre-veliger larvae was found to be mineralized, confirming that calcification occurred before larval torsion. Using both infra-red and Raman spectroscopy, we showed that CaCO3 deposition occurred at the pre-veliger stage and that the mineral phase initially deposited was essentially composed of aragonite.

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DO - 10.1007/s00227-008-0966-3

M3 - Article

AN - SCOPUS:44449098570

SN - 0025-3162

VL - 154

SP - 735

EP - 744

JO - Marine Biology

JF - Marine Biology

IS - 4

ER -

A morphological and structural study of the larval shell from the abalone Haliotis tuberculata

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