A mutation in the short 5'-proximal open reading frame on Rous sarcoma virus RNA alters virus production

R. B. Petersen, A. Moustakas, P. B. Hackett

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The 5'-proximal open reading frame on Rous sarcoma virus RNA encodes a seven-amino-acid peptide and is conserved in all avian sarcoma-leukosis retroviruses. Ribosome-binding site analysis in intact chick cells showed that the 5'-proximal AUG codon is a strong site for initiation of translation in vivo. Removal of the 5'-proximal AUG codon by site-specific mutagenesis resulted in a virus with a reduced ability either to replicate or to transform a population of chicken embryo fibroblasts. These results establish a procedure for determining sites of ribosome binding and initiation of translation on mRNAs in intact eucaryotic cells and strongly suggest that the 5'-proximal open reading frame (or its AUG codon) on Rous sarcoma virus RNA has an important role in regulating viral activity.

Original languageEnglish
Pages (from-to)4787-4796
Number of pages10
JournalJournal of Virology
Volume63
Issue number11
DOIs
StatePublished - 1989

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