A thin layer chromatography laboratory experiment of medical importance

Loretta Sharma, Ankur Desai, Ajit Sharma

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


A thin layer chromatography experiment of medical importance is described. The experiment involves extraction of lipids from simulated amniotic fluid samples followed by separation, detection, and scanning of the lecithin and sphingomyelin bands on TLC plates. The lecithin-to-sphingomyelin ratio is calculated. The clinical significance of this number is discussed. Since this is a procedure that is often performed in medical laboratories, most of the supplies and materials for the experiment are commercially available in kit form.

Original languageEnglish
Pages (from-to)44-48
Number of pages5
JournalBiochemistry and Molecular Biology Education
Issue number1
StatePublished - Jan 2006


  • Clinical chemistry
  • Lecithin
  • Sphingomyelin
  • TLC


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