A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

Jessica A. Stewart; Brent F. Piligian; Sarah R. Rundell; Benjamin M. Swarts

doi:10.1039/c5cc07536j

A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

Jessica A. Stewart, Brent F. Piligian, Sarah R. Rundell, Benjamin M. Swarts

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.

Original language	English
Pages (from-to)	17600-17603
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	99
DOIs	https://doi.org/10.1039/c5cc07536j
State	Published - Oct 15 2015

Access to Document

10.1039/c5cc07536j

Cite this

@article{77b5268229b54a4ea993f26bfcb19cb4,

title = "A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags",

abstract = "A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.",

author = "Stewart, {Jessica A.} and Piligian, {Brent F.} and Rundell, {Sarah R.} and Swarts, {Benjamin M.}",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2015",

month = oct,

day = "15",

doi = "10.1039/c5cc07536j",

language = "English",

volume = "51",

pages = "17600--17603",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Chemical Communications",

number = "99",

}

TY - JOUR

T1 - A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

AU - Stewart, Jessica A.

AU - Piligian, Brent F.

AU - Rundell, Sarah R.

AU - Swarts, Benjamin M.

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2015/10/15

Y1 - 2015/10/15

N2 - A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.

AB - A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.

UR - http://www.scopus.com/inward/record.url?scp=84948671631&partnerID=8YFLogxK

U2 - 10.1039/c5cc07536j

DO - 10.1039/c5cc07536j

M3 - Article

C2 - 26482224

AN - SCOPUS:84948671631

SN - 1359-7345

VL - 51

SP - 17600

EP - 17603

JO - Chemical Communications

JF - Chemical Communications

IS - 99

ER -

A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

Abstract

Access to Document

Other files and links

Fingerprint

Cite this