Acyl CoA and lipid synthesis from ketone bodies by the extramitochondrial fraction of hepatoma tissue

Leslie Hildebrandt, Terry Spennetta, Ronald Ackerman, Charles Elson, Earl Shrago

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The compartmentation of the pathway for the synthesis of lipids from ketone bodies was examined in a cell free, post-mitochondrial supernatant fraction of malignant Morris hepatoma 7777 tissue. A fortified supernatant system effectively incorporated radioactive D(-)-3-hydroxybulyrate and acetoacetate into cholesterol and fatty acids, and both ketone bodies were directly converted to their CoA thioesters. Furthermore, a microsome-free (100,000 x g) cytosolic fraction was also able to acylate 3-hydroxybutyrate to 3-hydroxy-butyryl CoA. No previous identification of this enzyme activity has been described. These results which characterize a distinct extramitochondrial pathway for conversion of 3-hydroxybutyrate as well as acetoacetate into lipids also suggest the possibility of a previously undetected enzymatic activity for utilization of this ketone body.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume225
Issue number1
DOIs
StatePublished - Aug 5 1996
Externally publishedYes

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