Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection

Michael J. Conway, Berlin Londono-Renteria, Andrea Troupin, Alan M. Watson, William B. Klimstra, Erol Fikrig, Tonya M. Colpitts

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48 Scopus citations


Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1–4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. Previously, we showed that Ae. aegypti salivary gland extracts (SGEs) enhanced dissemination of DENV to draining lymph nodes. In contrast, HPLC-fractionation revealed that some SGE components inhibited infection. Here, we show that D7 proteins are enriched in HPLC fractions that are inhibitory to DENV infection, and that recombinant D7 protein can inhibit DENV infection in vitro and in vivo. Further, binding assays indicate that D7 protein can directly interact with DENV virions and recombinant DENV envelope protein. These data reveal a novel role for D7 proteins, which inhibits arbovirus transmission to vertebrates through a direct interaction with virions.

Original languageEnglish
Article numbere0004941
JournalPLoS Neglected Tropical Diseases
Issue number9
StatePublished - Sep 15 2016


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