Aerosolized vaccine as an unexpected source of false-positive Bordetella pertussis PCR results

Hossein Salimnia, Paul R. Lephart, Basim I. Asmar, Dawn Prebelich, Erin Paulson, Marilynn R. Fairfax

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

When 13 of 13 nasal wash specimens from a single pediatrician's office tested positive for low quantities of Bordetella pertussis DNA, we suspected prelaboratory contamination. Investigation revealed that Pentacel and Adacel vaccines contain high copy numbers of B. pertussis DNA, which can be aerosolized, causing false-positive B. pertussis PCR results.

Original languageEnglish
Pages (from-to)472-474
Number of pages3
JournalJournal of Clinical Microbiology
Volume50
Issue number2
DOIs
StatePublished - Feb 2012

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