Abstract
OBJECTIVE: To explore the genetic polymorphism of HLA-DQA1 locus in Han nationality in Hunan province. METHOD: Sixty samples randomly selected from healthy individuals of Han nationality in Hunan province were typed for HLA-DQA1 by PCR/SSP in combination with silver-staining PCR/SSCP. RESULTS: Ten types of HLA-DQA1 alleles were found in all 60 samples, HLA-DQA1*0302, *0501, *0102 were the common alleles with gene frequencies of 0.2254, 0.2254, and 0.2041 respectively. Twenty-six kinds of genotypes were found, and the genotype distribution fitted the Hardy-Weinberg equilibrium test (P > 0.05). Five samples which had ambiguity in their PCR/SSP typing were further clarified with PCR/SSCP analysis. CONCLUSIONS: Normal gene frequency values of HLA-DQA1 alleles in Han nationality in Hunan province are provided; PCR/SSCP can be used to eliminate the ambiguity which might exist in PCR/SSP typing for HLA-DQA1 locus.
| Original language | English |
|---|---|
| Pages (from-to) | 141-143 |
| Number of pages | 3 |
| Journal | Bulletin of Hunan Medical University |
| Volume | 25 |
| Issue number | 2 |
| State | Published - Apr 28 2000 |
| Externally published | Yes |