Altered T cell cytokine production following mechanical trauma

Kathleen L. Meert, John P. Ofenstein, Ashok P. Sarnaik

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Background: Cell mediated immunity is suppressed following traumatic injury. The objective is to determine whether there is a shift from T helper type 1 (T(H)1) to T(H)2 cell cytokine production following mechanical trauma in a rat model. Methods: Male Sprague-Dawley rats were anesthetized and subjected to bilateral femur fractures or sham injury. Spleens were removed 3 days later. T cell proliferation and cytokine production were stimulated by culturing spleen cells with the T cell mitogen concanavalin A (con A). Interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-10 and IL-4 concentrations were measured in spleen cell supernatants using enzyme linked immunosorbent assays. Results: Con A-induced spleen cell proliferation was decreased in traumatized rats compared to controls (p < 0.05). Spleen cell supernatant concentrations of the T(H)1 cytokines IL-2 and IFN-γ were decreased in the trauma group (p < 0.05). Supernatant concentrations of the T(H)2 cytokine IL- 10 were also decreased in traumatized rats (p < 0.01). The IL-4 concentrations were below the detection limit (<15 pg/mL) in all cell supernatants. Conclusions: Mechanical tissue injury leads to generalized suppression of T helper cell cytokine production rather than a shift from T(H)l to T(H)2 cell activity. Post-trauma cellular immunosuppression is not mediated via excess IL-10 production by T(H)2 cells.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalAnnals of Clinical and Laboratory Science
Issue number5
StatePublished - 1998


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