Analysis of HLA-Cw, KIR2D genetic variations in 2 Chinese populations

Jin Hong Cai, Wei Tian, Li Xin Li, Fan Wang, Ke Zhu Sun, Qing Ren Zeng, Shi Shi Guo, Ya Cao

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To explore the genetic variations of HLA-Cw and 5 KIR2D loci in 2 Chinese Han populations residing at Southern and Northern mainland China, respectively, and to investigate the HLA-Cw polymorphism of a Mongolian Chinese population. Methods: HLA-Cw genotyping was performed in a total of 293 healthy individuals including 1 Southern Han population living in Hunan Province (n = 112), 1 Northern Han population (n = 98) and 1 Mongolian Chinese population(n = 83) in the Inner Mongolia Autonomous Region, using polymerase chain reaction-sequence specific primer(PCR-SSP) technique. Dimorphism at residue 80 of domain in the HLA-Cw molecule was examined by an additional set of PCR-SSP reactions. PCR-SSP was also used to detect the presence or absence of inhibitory KIR2DL1/2DL2/2DL3 loci and activating KIR2DS1/2DS2 loci for the 2 Han populations. Results: The main findings were: (1) Very significant frequency difference in the HLA-Cw alleles and dimorphism at codon 80 was detected between Hunan Han and Northern Han population, and between Hunan Han and Mongolian population (P < 0.001), while there was no such difference between the 2 Northern Chinese populations (P > 0.05); (2) There was no significant difference in frequencies of either the 5 individual KIR2D genes or the genotype distributions between the 2 Han populations (P > 0.05); (3) Asn 80/Asn80, 2DL1 + /2DL2-/2DL3 + /2DS1-/2DS2- predominated in both Han populations (45/112, 29/98), followed by ASn80/Asn 80, 2DL1 +/2DL2-/2DL3 + /2DS1 + /2DS2-(18/112, 16/98) and Asn 80/Lys80 2DL1 + / 2DL2-/2DL3 + /2DS1-/2DS2-(11/112, 17/98). Among the 12 types of HLA-Cw codon 80 and KIR2D combinations, only Lys80/Lys80, 2DL1 + /2DL2-/2DL3 + /2DS1-/2DS2- showed marginally significant frequency difference between the 2 Han populations(1/112 vs 8/98; Fisher's P = 0.0134). Conclusion: Our study provided the polymorphism data of HLA-Cw gene for 3 Chinese populations with different geographic and/or ethnic background, we further analyzed the distribution of 5 KIR2D receptor genes in 2 Han populations. Our data suggest that in spite of HLA-Cw heterogeneity, remarkable similarities may exist between the Southern and Northern Chinese Han populations at the combinational level of HLA-Cw and KIR2D, which are characterized by preponderant inhibitory signal pathways.

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalChinese Journal of Medical Genetics
Volume25
Issue number3
StatePublished - Jun 10 2008
Externally publishedYes

Keywords

  • Han ethnicity
  • Human leucocyte antigen-Cw
  • Killer immunoglobulin-like receptor
  • Mongol
  • Single nucleotide polymorphism

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