Application of tissue clearing and light sheet fluorescence microscopy to assess optic nerve regeneration in unsectioned tissues

Xueting Luo; Benjamin Yungher; Kevin K. Park

doi:10.1007/978-1-4939-0777-9_17

Application of tissue clearing and light sheet fluorescence microscopy to assess optic nerve regeneration in unsectioned tissues

Xueting Luo, Benjamin Yungher, Kevin K. Park

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.

Original language	English
Pages (from-to)	209-217
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1162
DOIs	https://doi.org/10.1007/978-1-4939-0777-9_17
State	Published - 2014
Externally published	Yes

Keywords

Axon regeneration
Light sheet fluorescence microscopy
Optical clearing
PTEN
Retinal ganglion cells
Tissue clearing
Ultramicroscope

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10.1007/978-1-4939-0777-9_17

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title = "Application of tissue clearing and light sheet fluorescence microscopy to assess optic nerve regeneration in unsectioned tissues",

abstract = "Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.",

keywords = "Axon regeneration, Light sheet fluorescence microscopy, Optical clearing, PTEN, Retinal ganglion cells, Tissue clearing, Ultramicroscope",

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AU - Yungher, Benjamin

AU - Park, Kevin K.

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PY - 2014

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N2 - Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.

AB - Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.

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KW - Light sheet fluorescence microscopy

KW - Optical clearing

KW - PTEN

KW - Retinal ganglion cells

KW - Tissue clearing

KW - Ultramicroscope

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ER -

Application of tissue clearing and light sheet fluorescence microscopy to assess optic nerve regeneration in unsectioned tissues

Abstract

Keywords

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