Background: Although there is mounting evidence of the benefits of probiotics, many consumers are unaware of the definition of probiotics and are unable to state which foods contain these live microorganisms. Purpose: This study attempted to determine if participants were able to state the definition of probiotics, whether they utilize these products regularly and if they would consider consuming probiotics if it were recommended by a health professional. Methods: The subjects were evaluated via an 18-item questionnaire. A total of 335 surveys were returned completed. Results: This study demonstrates that 38.5% of respondents had heard of probiotics, but only 27.2% stated that they knew what probiotics are. Those participants who stated they knew what probiotics are were more likely to try them when recommended. Participants who were able to state the benefit of probiotics, consumed them in greater frequency. Discussion: The results of this study support the hypothesis that many consumers are unaware of the definition of probiotics and are unable to state which foods contain these live microorganisms. Translation to Health Education Practice: These results indicate a need for further education of the general public in regards to the definition, benefits, and sources of probiotics.