C. elegans Dicer interacts with the P-granule component GLH-1 and both regulate germline RNPs

Erica L. Beshore, Tamara J. McEwen, Molly C. Jud, Jordan K. Marshall, Jennifer A. Schisa, Karen L. Bennett

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

P granules, ribonucleoprotein (RNP) complexes specific to the cytoplasmic side of the nuclear pores of Caenorhabditis elegans germ cells, are implicated in post-transcriptional control of maternally-transcribed mRNAs. Here we show a relationship in C. elegans of Dicer, the riboendonuclease processing enzyme of the RNA interference and microRNA pathways, with GLH-1, a germline-specific RNA helicase and a constitutive component of P granules. Based on results from GST-pull-downs and immunoprecipitations, GLH-1 binds DCR-1 and this binding does not require RNA. Both GLH-1 protein and glh-1 mRNA levels are reduced in the dcr-1(ok247) null mutant background; conversely, a reduction of DCR-1 protein is observed in the glh-1(gk100) deletion strain. Thus, in the C. elegans germline, DCR-1 and GLH-1 are interdependent. In addition, evidence indicates that DCR-1 protein levels, like those of GLH-1, are likely regulated by the Jun N-terminal kinase (JNK), KGB-1. In adult germ cells, DCR-1 is found in uniformly-distributed, small puncta both throughout the cytoplasm and the nucleus, on the inner side of nuclear pores, and associated with P granules. In arrested oocytes, GLH-1 and DCR-1 re-localize to cytoplasmic and cortically-distributed RNP granules and are necessary to recruit other components to these complexes. We predict that the GLH-1/DCR-1 complex may function in the transport, deposition, or regulation of maternally-transcribed mRNAs and their associated miRNAs.

Original languageEnglish
Pages (from-to)370-381
Number of pages12
JournalDevelopmental Biology
Volume350
Issue number2
DOIs
StatePublished - Feb 15 2011

Keywords

  • Jun N-terminal kinase (JNK)
  • KGB-1
  • MiRNA pathway
  • Oocyte RNP granules
  • Processing bodies

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