CD14/16 monocyte profiling in juvenile myelomonocytic leukemia

Manisha Gadgeel, Shruti Bagla, Steven Buck, Mark Shamoun, Yaddanapudi Ravindranath

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Monocyte subset analysis by flow cytometry has been shown to be a useful diagnostic tool in chronic myelomonocytic leukemia in adults. An increase in the classical monocyte fraction (CD14++/CD16−) greater than 94.0% of total monocytes is considered highly sensitive and specific in distinguishing chronic myelomonocytic leukemia from other myeloproliferative disorders. In a pilot study of juvenile myelomonocytic leukemia cases, we noted that CD14++/CD16− monocyte fraction was >95% in de novo juvenile myelomonocytic leukemia (JMML) with somatic PTPN11 mutations but normal in those with monosomy 7 or Noonan syndrome. Monocyte subgroup profiling by itself is not diagnostic of JMML but may distinguish molecular subgroups within JMML.

Original languageEnglish
Article numbere28555
JournalPediatric Blood and Cancer
Issue number9
StatePublished - Sep 1 2020


  • flow cytometry
  • juvenile myelomonocytic leukemia
  • monocyte subsets


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