TY - JOUR
T1 - Cell wall glycosphingolipids of Sphingomonas paucimobilis are CD1d-specific ligands for NKT cells
AU - Sriram, Venkataraman
AU - Du, Wenjun
AU - Gervay-Hague, Jacquelyn
AU - Brutkiewicz, Randy R.
PY - 2005/6
Y1 - 2005/6
N2 - The current consensus on characterization of NKT cells is based on their reactivity to the synthetic glycolipid, α-galactosylceramide (α-GalCer) in a CD1d-dependent manner. Because of the limited availability of α-GalCer, there is a constant search for CD1d-presented ligands that activate NKT cells. The α-anomericity of the carbohydrate is considered to be an important requisite for the CD1d-specific activation of NKT cells. The gram-negative, lipopolysaccharide-free bacterium Sphingomonas paucimobilis is known to contain glycosphingolipids (GSL) with α-anomeric sugars attached to the lipid chain. Here, we report that GSL extracted from this bacterium are able to stimulate NKT cells in a CD1d-specific manner. In addition, soluble CD1d-Ig dimers loaded with this lipid extract specifically bind to NKT cells (but not conventional T cells). Further studies on the S. paucimobilis GSL could potentially lead to other natural sources of CD1d-specific ligands useful for NKT cell analyses and aimed at identifying novel therapies for a variety of disease states.
AB - The current consensus on characterization of NKT cells is based on their reactivity to the synthetic glycolipid, α-galactosylceramide (α-GalCer) in a CD1d-dependent manner. Because of the limited availability of α-GalCer, there is a constant search for CD1d-presented ligands that activate NKT cells. The α-anomericity of the carbohydrate is considered to be an important requisite for the CD1d-specific activation of NKT cells. The gram-negative, lipopolysaccharide-free bacterium Sphingomonas paucimobilis is known to contain glycosphingolipids (GSL) with α-anomeric sugars attached to the lipid chain. Here, we report that GSL extracted from this bacterium are able to stimulate NKT cells in a CD1d-specific manner. In addition, soluble CD1d-Ig dimers loaded with this lipid extract specifically bind to NKT cells (but not conventional T cells). Further studies on the S. paucimobilis GSL could potentially lead to other natural sources of CD1d-specific ligands useful for NKT cell analyses and aimed at identifying novel therapies for a variety of disease states.
KW - Antigen presentation
KW - Glycosphingolipids
KW - NKT cell activation
UR - http://www.scopus.com/inward/record.url?scp=20844463676&partnerID=8YFLogxK
U2 - 10.1002/eji.200526157
DO - 10.1002/eji.200526157
M3 - Article
C2 - 15915536
AN - SCOPUS:20844463676
VL - 35
SP - 1692
EP - 1701
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 6
ER -