Characterization of a novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, by cloning and sequencing

W. Tian; F. M. Zhu; W. Y. Wang; L. X. Li; J. H. Cai

doi:10.1111/iji.12372

Characterization of a novel allelic variant in HLA-B40 lineage, HLA-B40:298:02, by cloning and sequencing

W. Tian, F. M. Zhu, W. Y. Wang, L. X. Li, J. H. Cai

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction–Sanger sequence-based typing (PCR–SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence-based typed subjects from the same population.

Original language	English
Pages (from-to)	143-145
Number of pages	3
Journal	International Journal of Immunogenetics
Volume	45
Issue number	3
DOIs	https://doi.org/10.1111/iji.12372
State	Published - Jun 2018
Externally published	Yes

Keywords

HLA-B*40:298:02
cloning
long-range PCR
sequence-based typing
southern Chinese Han population

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10.1111/iji.12372

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@article{d3e7244f0a28483c91bdef6921202418,

title = "Characterization of a novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, by cloning and sequencing",

abstract = "A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction–Sanger sequence-based typing (PCR–SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence-based typed subjects from the same population.",

keywords = "HLA-B*40:298:02, cloning, long-range PCR, sequence-based typing, southern Chinese Han population",

author = "W. Tian and Zhu, {F. M.} and Wang, {W. Y.} and Li, {L. X.} and Cai, {J. H.}",

note = "Funding Information: We thank the blood donor. This work was supported partly by grants from the Program for New Century Excellent Talents in University (Proj.No. NCET-08-0564) and Central South University (Proj. No.2013JSJJ038) awarded to Wei Tian. Funding Information: We thank the blood donor. This work was supported partly by grants from the Program for New Century Excellent Talents in University (Proj.No. NCET-08-0564) and Central South University (Proj.No.2013JSJJ038) awarded to Wei Tian. Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons Ltd",

year = "2018",

month = jun,

doi = "10.1111/iji.12372",

language = "English",

volume = "45",

pages = "143--145",

journal = "International Journal of Immunogenetics",

issn = "1744-3121",

number = "3",

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AU - Tian, W.

AU - Zhu, F. M.

AU - Wang, W. Y.

AU - Li, L. X.

AU - Cai, J. H.

N1 - Funding Information: We thank the blood donor. This work was supported partly by grants from the Program for New Century Excellent Talents in University (Proj.No. NCET-08-0564) and Central South University (Proj. No.2013JSJJ038) awarded to Wei Tian. Funding Information: We thank the blood donor. This work was supported partly by grants from the Program for New Century Excellent Talents in University (Proj.No. NCET-08-0564) and Central South University (Proj.No.2013JSJJ038) awarded to Wei Tian. Publisher Copyright: © 2018 John Wiley & Sons Ltd

PY - 2018/6

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N2 - A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction–Sanger sequence-based typing (PCR–SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence-based typed subjects from the same population.

AB - A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction–Sanger sequence-based typing (PCR–SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule. This new allele has an estimated frequency of 0.0002, in about 2,500 sequence-based typed subjects from the same population.

KW - HLA-B40:298:02

KW - cloning

KW - long-range PCR

KW - sequence-based typing

KW - southern Chinese Han population

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JO - International Journal of Immunogenetics

JF - International Journal of Immunogenetics

IS - 3

ER -

Characterization of a novel allelic variant in HLA-B40 lineage, HLA-B40:298:02, by cloning and sequencing

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Keywords

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