Characterization of a novel allelic variant in HLA-B*46:01 lineage, HLA-B*46:01:25, by cloning, phasing and sequencing

Li Xin Li, Wei Tian, Fa Ming Zhu, Wen Yi Wang, Fan Wang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A new allelic variant in HLA-B*46:01 lineage, HLA-B*46:01:25, has been identified in a male individual of Mongol ethnicity residing in northern China. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this novel variant was further confirmed by cloning, phasing and sequencing. HLA-B*46:01:25 differs from HLA-B*46:01:01 by a single synonymous T substitution at nucleotide position 137 (C - T) in exon 4, corresponding to codon 228 (ACC-ACT) of the mature HLA-B mRNA molecule.

Original languageEnglish
Pages (from-to)347-350
Number of pages4
JournalInternational Journal of Immunogenetics
Volume45
Issue number6
DOIs
StatePublished - Dec 2018
Externally publishedYes

Keywords

  • Chinese Mongolian population
  • HLA-B*46:01:25
  • cloning
  • long-range PCR
  • sequence-based typing

Fingerprint

Dive into the research topics of 'Characterization of a novel allelic variant in HLA-B*46:01 lineage, HLA-B*46:01:25, by cloning, phasing and sequencing'. Together they form a unique fingerprint.

Cite this