TY - JOUR
T1 - Characterization of the major histocompatibility complex class I chain-related gene B (MICB) polymorphism in a northern Chinese Han population
T2 - The identification of a new MICB allele, MICB *023
AU - Liu, Xue Xiang
AU - Tian, Wei
AU - Li, Li Xin
AU - Cai, Jin Hong
N1 - Funding Information:
Research funding was provided by a governmental grant by the Dutch National Programme for Improving Care for Older persons (ZonMw no. 313080201). The Medical Ethics Committee of the VU University medical center approved the study protocol.
PY - 2011/9
Y1 - 2011/9
N2 - Major histocompatibility complex class I chain-related gene B (MICB) has only been characterized for allelic variation in very few human populations. The MICB polymorphism remains largely unknown in Chinese populations. In this study, 104 healthy unrelated Han subjects recruited from central Inner Mongolia Autonomous Region, northern China, were investigated by sequence-based typing for MICB allelic variation, the association of MICB alleles with AluyMICB insertion/deletion dimorphism located in MICB intron 1, linkage disequilibrium of MICB with human leukocyte antigen (HLA)-B and MICA, and HLA-A-C-B-MICA-MICB haplotypic diversity. Ten kinds of MICB alleles were observed, among which MICB *005:02/010, MICB *002:01, and MICB *004:01 were the most frequent alleles with frequencies of 51.44, 16.35, and 11.54%, respectively. Significant linkage disequilibrium (LD) was observed for 9 of the 21 HLA-B-MICB haplotypes and 6 of the 17 MICA-MICB haplotypes with a frequency >1.5%. In particular, HLA-B *13:01 and HLA-B *13:02, both of which were frequently represented in this population, exhibited a distinct LD pattern with the MICB allele. A new MICB allele, MICB *023, was identified, which differed from MICB *005:02/010 by a single mutation of G to A at position 86 in exon 2, resulting in an amino acid change from arginine to histidine at codon 6. HLA-A *30-C *06-B *13:02-MICA *008:01-MICB *005:02/010 was the most common haplotype, with a frequency of 8.64% in this population. HLA-A *02-C *08-B *48-MICA *Del-MICB *009N demonstrated a frequency of 2.4% in this population. Our results provide for the first time data regarding the MICB genetic polymorphism in northern Chinese Han populations and will form the basis for future studies of the potential role of MICB in allogeneic organ transplantation and disease association in related ethnic groups.
AB - Major histocompatibility complex class I chain-related gene B (MICB) has only been characterized for allelic variation in very few human populations. The MICB polymorphism remains largely unknown in Chinese populations. In this study, 104 healthy unrelated Han subjects recruited from central Inner Mongolia Autonomous Region, northern China, were investigated by sequence-based typing for MICB allelic variation, the association of MICB alleles with AluyMICB insertion/deletion dimorphism located in MICB intron 1, linkage disequilibrium of MICB with human leukocyte antigen (HLA)-B and MICA, and HLA-A-C-B-MICA-MICB haplotypic diversity. Ten kinds of MICB alleles were observed, among which MICB *005:02/010, MICB *002:01, and MICB *004:01 were the most frequent alleles with frequencies of 51.44, 16.35, and 11.54%, respectively. Significant linkage disequilibrium (LD) was observed for 9 of the 21 HLA-B-MICB haplotypes and 6 of the 17 MICA-MICB haplotypes with a frequency >1.5%. In particular, HLA-B *13:01 and HLA-B *13:02, both of which were frequently represented in this population, exhibited a distinct LD pattern with the MICB allele. A new MICB allele, MICB *023, was identified, which differed from MICB *005:02/010 by a single mutation of G to A at position 86 in exon 2, resulting in an amino acid change from arginine to histidine at codon 6. HLA-A *30-C *06-B *13:02-MICA *008:01-MICB *005:02/010 was the most common haplotype, with a frequency of 8.64% in this population. HLA-A *02-C *08-B *48-MICA *Del-MICB *009N demonstrated a frequency of 2.4% in this population. Our results provide for the first time data regarding the MICB genetic polymorphism in northern Chinese Han populations and will form the basis for future studies of the potential role of MICB in allogeneic organ transplantation and disease association in related ethnic groups.
KW - HLA
KW - Linkage disequilibrium
KW - MICB
KW - Northern Chinese Han population
KW - Sequence-based typing
UR - http://www.scopus.com/inward/record.url?scp=84860390190&partnerID=8YFLogxK
U2 - 10.1016/j.humimm.2011.05.013
DO - 10.1016/j.humimm.2011.05.013
M3 - Article
C2 - 21664939
AN - SCOPUS:84860390190
VL - 72
SP - 727
EP - 732
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 9
ER -