TY - JOUR
T1 - Combinational IL-2/IL-15 induction does not further enhance IL-15-induced lymphokine-activated killer cell cytotoxicity against human leukemia/lymphoma cells
AU - Özdemir, Öner
AU - Savaşan, Süreyya
N1 - Funding Information:
The authors are thankful to Michael Long and Steven Buck for their assistance in performing CRA and initial flow-based intracellular staining procedures, respectively. This study was supported partially by funds from Leukemia Research Life, LRL, Inc., Children's Research Center of Michigan, Detroit, MI.
PY - 2005/6
Y1 - 2005/6
N2 - In vitro comparisons of induction of perforin (PFP), granzyme B (GRB), production of cytokines, and cell-mediated cytotoxicity by interleukin-2 (IL-2), interleukin-15 (IL-15), or combinational IL-2/IL-15-induced lymphokine-activated killer cells were studied in this study. Whereas IL-2-induction was associated with a decrease in cultured cell population over a 7-day period, IL-15 alone or in combination with IL-2 resulted in significant increase including cytotoxic T lymphocytes and subsets of CD56+ lymphocytes, particularly cytokine-induced killer and cytolytic natural killer-T lymphocytes. The overall PFP, GRB, and tumor necrosis factor-α expression in different subtypes were also significantly higher with IL-15 alone or in combination with IL-2 induction with resultant superior cytotoxicity compared to IL-2 treatment. There was no significant advantage of addition of IL-2 over IL-15 induction. These results offer further information on the cytotoxic potency of these cytokines and their mechanisms of action implicating potential use of IL-15 as part of cytokine adoptive immunotherapy.
AB - In vitro comparisons of induction of perforin (PFP), granzyme B (GRB), production of cytokines, and cell-mediated cytotoxicity by interleukin-2 (IL-2), interleukin-15 (IL-15), or combinational IL-2/IL-15-induced lymphokine-activated killer cells were studied in this study. Whereas IL-2-induction was associated with a decrease in cultured cell population over a 7-day period, IL-15 alone or in combination with IL-2 resulted in significant increase including cytotoxic T lymphocytes and subsets of CD56+ lymphocytes, particularly cytokine-induced killer and cytolytic natural killer-T lymphocytes. The overall PFP, GRB, and tumor necrosis factor-α expression in different subtypes were also significantly higher with IL-15 alone or in combination with IL-2 induction with resultant superior cytotoxicity compared to IL-2 treatment. There was no significant advantage of addition of IL-2 over IL-15 induction. These results offer further information on the cytotoxic potency of these cytokines and their mechanisms of action implicating potential use of IL-15 as part of cytokine adoptive immunotherapy.
KW - Cell-mediated cytotoxicity
KW - Cytotoxic NK-T cell
KW - Flow cytometric cell-mediated cytotoxicity assay
KW - Granzyme B
KW - IL-2 and IL-15 combination
KW - Interferon-γ
KW - Leukemia/lymphoma cells
KW - NK/LAK activity
KW - Perforin
KW - Tumor necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=19044364601&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2005.01.008
DO - 10.1016/j.clim.2005.01.008
M3 - Article
C2 - 15893691
AN - SCOPUS:19044364601
VL - 115
SP - 240
EP - 249
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 3
ER -