Comparative analysis of Lake Periphyton communities using high performance liquid chromatography (HPLC) and light microscope counts

Karl E. Havens; Alan D. Steinman; H. J. Carrick; J. William Louda; Nancy M. Winfree; Earl W. Baker

doi:10.1007/s000270050068

Comparative analysis of Lake Periphyton communities using high performance liquid chromatography (HPLC) and light microscope counts

Karl E. Havens, Alan D. Steinman, H. J. Carrick, J. William Louda, Nancy M. Winfree, Earl W. Baker

Biology

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18 Scopus citations

Abstract

High performance liquid chromatography (HPLC) and light microscope counts were used to characterize the taxonomic composition of epiphyton and epipelon at seven locations in a subtropical lake. Both methods indicated that algae were dominated by diatoms and cyanobacteria. However, the methods often gave dramatically different estimates of relative biomass among algal divisions, and there was no consistent pattern of co-variation. Large differences in underwater irradiance may have caused variation in accessory pigment to chlorophyll a ratios, invalidating application of the generic HPLC-based model. In large heterogeneous lakes, it may be necessary to use a suite of models, tailored to site-specific environmental conditions, if HPLC is to be used for evaluation of algal community structure.

Original language	English
Pages (from-to)	307-322
Number of pages	16
Journal	Aquatic Sciences
Volume	61
Issue number	4
DOIs	https://doi.org/10.1007/s000270050068
State	Published - 1999

Keywords

HPLC
Light microscopy
Periphyton
Pigment analysis

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10.1007/s000270050068

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title = "Comparative analysis of Lake Periphyton communities using high performance liquid chromatography (HPLC) and light microscope counts",

abstract = "High performance liquid chromatography (HPLC) and light microscope counts were used to characterize the taxonomic composition of epiphyton and epipelon at seven locations in a subtropical lake. Both methods indicated that algae were dominated by diatoms and cyanobacteria. However, the methods often gave dramatically different estimates of relative biomass among algal divisions, and there was no consistent pattern of co-variation. Large differences in underwater irradiance may have caused variation in accessory pigment to chlorophyll a ratios, invalidating application of the generic HPLC-based model. In large heterogeneous lakes, it may be necessary to use a suite of models, tailored to site-specific environmental conditions, if HPLC is to be used for evaluation of algal community structure.",

keywords = "HPLC, Light microscopy, Periphyton, Pigment analysis",

author = "Havens, {Karl E.} and Steinman, {Alan D.} and Carrick, {H. J.} and Louda, {J. William} and Winfree, {Nancy M.} and Baker, {Earl W.}",

year = "1999",

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T1 - Comparative analysis of Lake Periphyton communities using high performance liquid chromatography (HPLC) and light microscope counts

AU - Havens, Karl E.

AU - Steinman, Alan D.

AU - Carrick, H. J.

AU - Louda, J. William

AU - Winfree, Nancy M.

AU - Baker, Earl W.

PY - 1999

Y1 - 1999

N2 - High performance liquid chromatography (HPLC) and light microscope counts were used to characterize the taxonomic composition of epiphyton and epipelon at seven locations in a subtropical lake. Both methods indicated that algae were dominated by diatoms and cyanobacteria. However, the methods often gave dramatically different estimates of relative biomass among algal divisions, and there was no consistent pattern of co-variation. Large differences in underwater irradiance may have caused variation in accessory pigment to chlorophyll a ratios, invalidating application of the generic HPLC-based model. In large heterogeneous lakes, it may be necessary to use a suite of models, tailored to site-specific environmental conditions, if HPLC is to be used for evaluation of algal community structure.

AB - High performance liquid chromatography (HPLC) and light microscope counts were used to characterize the taxonomic composition of epiphyton and epipelon at seven locations in a subtropical lake. Both methods indicated that algae were dominated by diatoms and cyanobacteria. However, the methods often gave dramatically different estimates of relative biomass among algal divisions, and there was no consistent pattern of co-variation. Large differences in underwater irradiance may have caused variation in accessory pigment to chlorophyll a ratios, invalidating application of the generic HPLC-based model. In large heterogeneous lakes, it may be necessary to use a suite of models, tailored to site-specific environmental conditions, if HPLC is to be used for evaluation of algal community structure.

KW - HPLC

KW - Light microscopy

KW - Periphyton

KW - Pigment analysis

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JO - Aquatic Sciences

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ER -

Comparative analysis of Lake Periphyton communities using high performance liquid chromatography (HPLC) and light microscope counts

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Keywords

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