Counter-current chromatography based analysis of synergy in an anti-tuberculosis ethnobotanical

Taichi Inui, Yuehong Wang, Shixin Deng, David C. Smith, Scott G. Franzblau, Guido F. Pauli

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The crude extract of an Alaskan ethnobotanical plant, Oplopanax horridus, was subjected to counter-current chromatography (CCC), and the selected active regions were evaluated for their synergistic effects with an in vitro model of anti-tubercular efficacy. CCC as a support-free high-resolution separation method was employed to preclude potential irreversible absorption to a solid stationary phase. The microplate Alamar blue assay and the isobole method were used to measure the biological activity and eliminate dose-response dependent errors, respectively. Using the combination of CCC, bioassay and isobole method, significant synergistic effects were observed. Among the entire polarity range, fractions with distribution constant between 0.44 and 0.81 showed the most synergistic enhancement with an increase in potency by 108% for the recombined fractions.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalJournal of Chromatography A
Volume1151
Issue number1-2
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

Keywords

  • CCC
  • Devil's Club
  • Isobole method
  • Oplopanax horridus
  • Synergy

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