TY - JOUR
T1 - Cre-dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice
AU - Zhu, Hu
AU - Aryal, Dipendra K.
AU - Olsen, Reid H.J.
AU - Urban, Daniel J.
AU - Swearingen, Amanda
AU - Forbes, Stacy
AU - Roth, Bryan L.
AU - Hochgeschwender, Ute
N1 - Funding Information:
Supported by NIH (UH), UO1MH104974 (BLR), and NIH NINDS National Research Service Award (F31NS093917-01) to R.H.J.O.
Publisher Copyright:
© 2016 Wiley Periodicals, Inc.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein-coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic β-cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology, we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase-mediated restricted expression of these pathway-selective DREADDs. With the many Cre driver lines now available, these DREADD lines will be applicable to studying a wide array of research and preclinical questions. genesis 54:439–446, 2016.
AB - DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein-coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic β-cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology, we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase-mediated restricted expression of these pathway-selective DREADDs. With the many Cre driver lines now available, these DREADD lines will be applicable to studying a wide array of research and preclinical questions. genesis 54:439–446, 2016.
KW - G protein-coupled receptors
KW - GsD
KW - chemogenetic
KW - hM3Dq
KW - hM4Di
UR - http://www.scopus.com/inward/record.url?scp=84983087278&partnerID=8YFLogxK
U2 - 10.1002/dvg.22949
DO - 10.1002/dvg.22949
M3 - Article
C2 - 27194399
AN - SCOPUS:84983087278
SN - 1526-954X
VL - 54
SP - 439
EP - 446
JO - Genesis
JF - Genesis
IS - 8
ER -
