Curcumin loaded dendrimers specifically reduce viability of glioblastoma cell lines

John Gallien, Bhairavi Srinageshwar, Kellie Gallo, Gretchen Holtgrefe, Sindhuja Koneru, Paulina Sequeiros Otero, Catalina Alvarez Bueno, Jamie Mosher, Alison Roh, D. Stave Kohtz, Douglas Swanson, Ajit Sharma, Gary Dunbar, Julien Rossignol

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Glioblastoma (GB) is a deadly and aggressive cancer of the CNS. Even with extensive resection and chemoradiotherapy, patient survival is still only 15 months. To maintain growth and proliferation, cancer cells require a high oxidative state. Curcumin, a well-known anti-inflammatory antioxidant, is a potential candidate for treatment of GB. To facilitate efficient delivery of therapeutic doses of curcumin into cells, we encapsulated the drug in surface-modified polyamidoamine (PAMAM) dendrimers. We studied the in vitro effectiveness of a traditional PAMAM dendrimer (100% amine surface, G4 NH2), surface-modified dendrimer (10% amine and 90% hydroxyl-G4 90/10-Cys), and curcumin (Cur)-encapsulated dendrimer (G4 90/10-Cys-Cur) on three species of glioblastoma cell lines: mouse-GL261, rat-F98, and human-U87. Using an MTT assay for cell viabil-ity, we found that G4 90/10-Cys-Cur reduced viability of all three glioblastoma cell lines compared to non-cancerous control cells. Under similar conditions, unencapsulated curcumin was not effec-tive, while the non-modified dendrimer (G4 NH2) caused significant death of both cancerous and normal cells. By harnessing and optimizing the components of PAMAM dendrimers, we are provid-ing a promising new route for delivering cancer therapeutics. Our results with curcumin suggest that antioxidants are good candidates for treating glioblastoma.

Original languageEnglish
Article number6050
JournalMolecules
Volume26
Issue number19
DOIs
StatePublished - Oct 1 2021

Keywords

  • Cancer
  • Curcumin
  • Glioblastoma
  • Nano-molecule
  • PAMAM dendrimers
  • Therapy
  • Toxicology

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