TY - JOUR
T1 - Detection of myocardial viability based on measurement of sodium content
T2 - A 23Na-NMR study
AU - Horn, Michael
AU - Weidensteiner, Claudia
AU - Scheffer, Heike
AU - Meininger, Martin
AU - De Groot, Mark
AU - Remkes, Helga
AU - Dienesch, Charlotte
AU - Przyklenk, Karin
AU - Von Kienlin, Markus
AU - Neubauer, Stefan
PY - 2001
Y1 - 2001
N2 - MRI of total sodium (Na) content may allow assessment of myocardial viability, but information on Na content in normal myocardium, necrotic/scar tissue, and stunned or hibernating myocardium is lacking. Thus, the aims of the study were to: 1) quantify the temporal changes in myocardial Na content postmyocardial infarction (MI) in a rat model (Protocol 1); 2) compare Na in normally perfused, hibernating, and stunned canine myocardium (Protocol 2); and 3) determine whether, in bufferperfused rat hearts, infarct scar can be differentiated from intact myocardium by 23Na-MRI (Protocol 3). In Protocol 1, rats were subjected to LAD ligation. Infarct/scar tissue was excised at control and 1, 3, 7, 28, 56, and 128 days post-MI (N = 6-8 each), Na content was determined by 23Na-NMR spectroscopy (MRS) and ion chromatography. Na content was persistently increased at all time points post-MI averaging 306*-160*% of control values (*P < 0.0083 vs. control). In Protocol 2, 23Na-MRS of control (baseline), stunned and hibernating samples revealed no difference in Na. In Protocol 3, 23Na-MRI revealed a mean increase in signal intensity, to 142 ± 6% of control values, in scar tissue. A threshold of 2 standard deviations of the image intensity allowed determination of infarct size, correlating with histologically determined infarct size (r = 0.91, P < 0.0001).
AB - MRI of total sodium (Na) content may allow assessment of myocardial viability, but information on Na content in normal myocardium, necrotic/scar tissue, and stunned or hibernating myocardium is lacking. Thus, the aims of the study were to: 1) quantify the temporal changes in myocardial Na content postmyocardial infarction (MI) in a rat model (Protocol 1); 2) compare Na in normally perfused, hibernating, and stunned canine myocardium (Protocol 2); and 3) determine whether, in bufferperfused rat hearts, infarct scar can be differentiated from intact myocardium by 23Na-MRI (Protocol 3). In Protocol 1, rats were subjected to LAD ligation. Infarct/scar tissue was excised at control and 1, 3, 7, 28, 56, and 128 days post-MI (N = 6-8 each), Na content was determined by 23Na-NMR spectroscopy (MRS) and ion chromatography. Na content was persistently increased at all time points post-MI averaging 306*-160*% of control values (*P < 0.0083 vs. control). In Protocol 2, 23Na-MRS of control (baseline), stunned and hibernating samples revealed no difference in Na. In Protocol 3, 23Na-MRI revealed a mean increase in signal intensity, to 142 ± 6% of control values, in scar tissue. A threshold of 2 standard deviations of the image intensity allowed determination of infarct size, correlating with histologically determined infarct size (r = 0.91, P < 0.0001).
KW - 3D-CSI
KW - Hibernating
KW - Myocardial infarction
KW - Ne NMR
KW - Stunning
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=0035039487&partnerID=8YFLogxK
U2 - 10.1002/mrm.1103
DO - 10.1002/mrm.1103
M3 - Article
C2 - 11323801
AN - SCOPUS:0035039487
VL - 45
SP - 756
EP - 764
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
SN - 0740-3194
IS - 5
ER -