Determination of fusaric acid in maize using molecularly imprinted SPE clean-up

Michael Appell, Michael A. Jackson, Lijuan C. Wang, Che Hin Ho, Anja Mueller

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

A new LC method to detect fusaric acid (FA) in maize is reported based on a molecularly imprinted SPE clean-up using mimic-templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or 2-dimethylaminoethyl methacrylate exhibited imprinting effects in SPE analysis. FA levels were determined using RP ion-pairing chromatography with diode-array UV detection and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect FA in maize using molecularly imprinted SPE analysis within the range of 1-100 μg/g with recoveries between 83.9 and 92.1%.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalJournal of Separation Science
Volume37
Issue number3
DOIs
StatePublished - Feb 2014

Keywords

  • Food safety
  • HPLC
  • Ion pair chromatography
  • Molecularly imprinted polymers
  • Mycotoxins

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