Distribution and abundance of Gram-positive bacteria in the environment: Development of a group-specific probe

Barbara J. MacGregor; Simon Toze; Elizabeth W. Alm; Richard Sharp; Cherie J. Ziemer; David A. Stahl

doi:10.1016/S0167-7012(00)00243-8

Distribution and abundance of Gram-positive bacteria in the environment: Development of a group-specific probe

Barbara J. MacGregor, Simon Toze, Elizabeth W. Alm, Richard Sharp, Cherie J. Ziemer, David A. Stahl

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12 Scopus citations

Abstract

We developed a 16S rRNA-targeted oligonucleotide probe (S-P-GPos-1200-a-A-13) for the Gram-positive bacteria, confirmed its specificity by database searches and hybridization studies, and investigated the effects of humic acids on membrane hybridizations with this probe. S-P-GPos-1200-a-A-13 was used to estimate the abundance of Gram-positive populations in the bovine rumen and Lake Michigan sediments. This probe should be useful for studies of the environmental distribution of Gram-positive bacteria and the detection of uncultured, phylogenetically Gram-positive bacteria with variable or negative Gram staining reactions, and could serve for Gram staining in some diagnostic settings.

Original language	English
Pages (from-to)	193-203
Number of pages	11
Journal	Journal of Microbiological Methods
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/S0167-7012(00)00243-8
State	Published - Apr 2 2001

Keywords

16S rRNA
Gram variable bacteria
Gram-positive bacteria
Humic acid
Lake Michigan
Oligonucleotide hybridization probe
Rumen

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10.1016/S0167-7012(00)00243-8

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title = "Distribution and abundance of Gram-positive bacteria in the environment: Development of a group-specific probe",

abstract = "We developed a 16S rRNA-targeted oligonucleotide probe (S-P-GPos-1200-a-A-13) for the Gram-positive bacteria, confirmed its specificity by database searches and hybridization studies, and investigated the effects of humic acids on membrane hybridizations with this probe. S-P-GPos-1200-a-A-13 was used to estimate the abundance of Gram-positive populations in the bovine rumen and Lake Michigan sediments. This probe should be useful for studies of the environmental distribution of Gram-positive bacteria and the detection of uncultured, phylogenetically Gram-positive bacteria with variable or negative Gram staining reactions, and could serve for Gram staining in some diagnostic settings.",

keywords = "16S rRNA, Gram variable bacteria, Gram-positive bacteria, Humic acid, Lake Michigan, Oligonucleotide hybridization probe, Rumen",

author = "MacGregor, {Barbara J.} and Simon Toze and Alm, {Elizabeth W.} and Richard Sharp and Ziemer, {Cherie J.} and Stahl, {David A.}",

note = "Funding Information: This work was supported by NSF Grant DEB-9615356. The Lake Michigan sediment samples were collected during cruises on the R/V Neeskay, for which we thank Captain Ron Smith, First Mate Greg Stamatelakys, Duane Moser, and the crew. We also thank the Center for Great Lakes Studies of the University of Wisconsin-Milwaukee for laboratory facilities.",

year = "2001",

month = apr,

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doi = "10.1016/S0167-7012(00)00243-8",

language = "English",

volume = "44",

pages = "193--203",

journal = "Journal of Microbiological Methods",

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TY - JOUR

T1 - Distribution and abundance of Gram-positive bacteria in the environment

T2 - Development of a group-specific probe

AU - MacGregor, Barbara J.

AU - Toze, Simon

AU - Alm, Elizabeth W.

AU - Sharp, Richard

AU - Ziemer, Cherie J.

AU - Stahl, David A.

N1 - Funding Information: This work was supported by NSF Grant DEB-9615356. The Lake Michigan sediment samples were collected during cruises on the R/V Neeskay, for which we thank Captain Ron Smith, First Mate Greg Stamatelakys, Duane Moser, and the crew. We also thank the Center for Great Lakes Studies of the University of Wisconsin-Milwaukee for laboratory facilities.

PY - 2001/4/2

Y1 - 2001/4/2

N2 - We developed a 16S rRNA-targeted oligonucleotide probe (S-P-GPos-1200-a-A-13) for the Gram-positive bacteria, confirmed its specificity by database searches and hybridization studies, and investigated the effects of humic acids on membrane hybridizations with this probe. S-P-GPos-1200-a-A-13 was used to estimate the abundance of Gram-positive populations in the bovine rumen and Lake Michigan sediments. This probe should be useful for studies of the environmental distribution of Gram-positive bacteria and the detection of uncultured, phylogenetically Gram-positive bacteria with variable or negative Gram staining reactions, and could serve for Gram staining in some diagnostic settings.

AB - We developed a 16S rRNA-targeted oligonucleotide probe (S-P-GPos-1200-a-A-13) for the Gram-positive bacteria, confirmed its specificity by database searches and hybridization studies, and investigated the effects of humic acids on membrane hybridizations with this probe. S-P-GPos-1200-a-A-13 was used to estimate the abundance of Gram-positive populations in the bovine rumen and Lake Michigan sediments. This probe should be useful for studies of the environmental distribution of Gram-positive bacteria and the detection of uncultured, phylogenetically Gram-positive bacteria with variable or negative Gram staining reactions, and could serve for Gram staining in some diagnostic settings.

KW - 16S rRNA

KW - Gram variable bacteria

KW - Gram-positive bacteria

KW - Humic acid

KW - Lake Michigan

KW - Oligonucleotide hybridization probe

KW - Rumen

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U2 - 10.1016/S0167-7012(00)00243-8

DO - 10.1016/S0167-7012(00)00243-8

M3 - Article

C2 - 11240041

AN - SCOPUS:0035795211

SN - 0167-7012

VL - 44

SP - 193

EP - 203

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Distribution and abundance of Gram-positive bacteria in the environment: Development of a group-specific probe

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