Distribution and abundance of Gram-positive bacteria in the environment: development of a group-specific probe

B J MacGregor, Elizabeth Wheeler Alm

Research output: Contribution to journalArticlepeer-review

Abstract

We developed a 16S rRNA-targeted oligonucleotide probe (S-P-GPos-1200-a-A-13) for the Gram-positive bacteria, confirmed its specificity by database searches and hybridization studies, and investigated the effects of humic acids on membrane hybridizations with this probe. S-P-GPos-1200-a-A-13 was used to estimate the abundance of Gram-positive populations in the bovine rumen and Lake Michigan sediments. This probe should be useful for studies of the environmental distribution of Gram-positive bacteria and the detection of uncultured, phylogenetically Gram-positive bacteria with variable or negative Gram staining reactions, and could serve for Gram staining in some diagnostic settings.

Original languageEnglish
Pages (from-to)193-203
JournalJournal of Microbiological Methods
Volume44
StatePublished - 2001

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