Donor cell chimerism evaluation in cerebrospinal fluid by short tandem repeat analysis following allogeneic hematopoietic stem cell transplantation

Jessica L. Spruit; Omar R. Fagoaga; Süreyya Savaşan

doi:10.1016/j.humimm.2018.04.001

Donor cell chimerism evaluation in cerebrospinal fluid by short tandem repeat analysis following allogeneic hematopoietic stem cell transplantation

Jessica L. Spruit, Omar R. Fagoaga, Süreyya Savaşan

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Cerebrospinal fluid (CSF) cell count, morphology and flow cytometric evaluations are used to investigate central nervous system (CNS) involvement in leukemia/lymphoma. We performed CSF short tandem repeat (STR) analysis to monitor CSF chimerism status and evaluate for disease involvement in 11 asymptomatic pediatric allogeneic hematopoietic stem cell transplantation (HSCT) recipients with hemato-lymphoid neoplasms and high risk for or history of CNS involvement. Eighteen (64%) of the samples with median CSF cell count of 1/mm³ with 90% lymphocytes gave conclusive STR results, suggesting that this DNA-based method can be used in monitoring CSF chimerism status after HSCT with an acceptable yield.

Original language	English
Pages (from-to)	537-538
Number of pages	2
Journal	Human Immunology
Volume	79
Issue number	7
DOIs	https://doi.org/10.1016/j.humimm.2018.04.001
State	Published - Jul 2018

Keywords

Cerebrospinal fluid
Donor chimerism
Hematopoietic stem cell transplantation
Short tandem repeat analysis

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10.1016/j.humimm.2018.04.001

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title = "Donor cell chimerism evaluation in cerebrospinal fluid by short tandem repeat analysis following allogeneic hematopoietic stem cell transplantation",

abstract = "Cerebrospinal fluid (CSF) cell count, morphology and flow cytometric evaluations are used to investigate central nervous system (CNS) involvement in leukemia/lymphoma. We performed CSF short tandem repeat (STR) analysis to monitor CSF chimerism status and evaluate for disease involvement in 11 asymptomatic pediatric allogeneic hematopoietic stem cell transplantation (HSCT) recipients with hemato-lymphoid neoplasms and high risk for or history of CNS involvement. Eighteen (64%) of the samples with median CSF cell count of 1/mm3 with 90% lymphocytes gave conclusive STR results, suggesting that this DNA-based method can be used in monitoring CSF chimerism status after HSCT with an acceptable yield.",

keywords = "Cerebrospinal fluid, Donor chimerism, Hematopoietic stem cell transplantation, Short tandem repeat analysis",

author = "Spruit, {Jessica L.} and Fagoaga, {Omar R.} and S{\"u}reyya Sava{\c s}an",

note = "Publisher Copyright: {\textcopyright} 2018 American Society for Histocompatibility and Immunogenetics",

year = "2018",

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T1 - Donor cell chimerism evaluation in cerebrospinal fluid by short tandem repeat analysis following allogeneic hematopoietic stem cell transplantation

AU - Spruit, Jessica L.

AU - Fagoaga, Omar R.

AU - Savaşan, Süreyya

PY - 2018/7

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N2 - Cerebrospinal fluid (CSF) cell count, morphology and flow cytometric evaluations are used to investigate central nervous system (CNS) involvement in leukemia/lymphoma. We performed CSF short tandem repeat (STR) analysis to monitor CSF chimerism status and evaluate for disease involvement in 11 asymptomatic pediatric allogeneic hematopoietic stem cell transplantation (HSCT) recipients with hemato-lymphoid neoplasms and high risk for or history of CNS involvement. Eighteen (64%) of the samples with median CSF cell count of 1/mm3 with 90% lymphocytes gave conclusive STR results, suggesting that this DNA-based method can be used in monitoring CSF chimerism status after HSCT with an acceptable yield.

AB - Cerebrospinal fluid (CSF) cell count, morphology and flow cytometric evaluations are used to investigate central nervous system (CNS) involvement in leukemia/lymphoma. We performed CSF short tandem repeat (STR) analysis to monitor CSF chimerism status and evaluate for disease involvement in 11 asymptomatic pediatric allogeneic hematopoietic stem cell transplantation (HSCT) recipients with hemato-lymphoid neoplasms and high risk for or history of CNS involvement. Eighteen (64%) of the samples with median CSF cell count of 1/mm3 with 90% lymphocytes gave conclusive STR results, suggesting that this DNA-based method can be used in monitoring CSF chimerism status after HSCT with an acceptable yield.

KW - Cerebrospinal fluid

KW - Donor chimerism

KW - Hematopoietic stem cell transplantation

KW - Short tandem repeat analysis

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JO - Human Immunology

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ER -

Donor cell chimerism evaluation in cerebrospinal fluid by short tandem repeat analysis following allogeneic hematopoietic stem cell transplantation

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