Abstract
Objective: To study the expression of MHC class I molecules and MHC class II molecules on HeLa cells infected with Chlamydia trachomatis. Methods: The immune fluorescence assay and flow cytometer assay were adopted to analyze the expression of MHC class I molecules and MHC class II molecules induced by IFN-γ on the infected and uninfected HeLa cells. The antagonistic action of anti-IL-10 to IL-10 was assyed. Results: The expression of MHC class I and IFN-γ-induced MHC class II molecules on C. trachomatis-infected HeLa cells were decreased with increasing dose of Ct and infection time. The differences mentioned above were all significant as compared with corresponding control cells (P < 0.01). IL-10 antibody inhibited partially the down-regulation of MHC class I, but not IFN-γ-induced MHC class II molecules expression, on infected HeLa cells. Conclusion: The results suggest that Ct infection down-regulate MHC class I molecules and IFN-γ-induced MHC class II molecules expression on HeLa cells which may facilitate chlamydial persistence. The inhibition of MHC class I, but not IFN-γ-induced MHC class II molecules expression on HeLa cells infected with C. trachomatis is due, at least partially, to the IL-10 induced during C. trachomatis infection.
Original language | English |
---|---|
Pages (from-to) | 44-47 |
Number of pages | 4 |
Journal | Chinese Journal of Microbiology and Immunology |
Volume | 24 |
Issue number | 1 |
State | Published - Jan 2004 |
Externally published | Yes |
Keywords
- Chlamydia trachomatis
- HeLa cell
- IL-10 antibody
- Major histocompatibility complex class I and II molecules