Effect of Chlamydia trachomatis infection on the MHC class I and II molecules expression on HeLa cells

Jun Long Yu, Fa Qing Tang, Li Xin Li, Ming Xing Shu, Ping Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To study the expression of MHC class I molecules and MHC class II molecules on HeLa cells infected with Chlamydia trachomatis. Methods: The immune fluorescence assay and flow cytometer assay were adopted to analyze the expression of MHC class I molecules and MHC class II molecules induced by IFN-γ on the infected and uninfected HeLa cells. The antagonistic action of anti-IL-10 to IL-10 was assyed. Results: The expression of MHC class I and IFN-γ-induced MHC class II molecules on C. trachomatis-infected HeLa cells were decreased with increasing dose of Ct and infection time. The differences mentioned above were all significant as compared with corresponding control cells (P < 0.01). IL-10 antibody inhibited partially the down-regulation of MHC class I, but not IFN-γ-induced MHC class II molecules expression, on infected HeLa cells. Conclusion: The results suggest that Ct infection down-regulate MHC class I molecules and IFN-γ-induced MHC class II molecules expression on HeLa cells which may facilitate chlamydial persistence. The inhibition of MHC class I, but not IFN-γ-induced MHC class II molecules expression on HeLa cells infected with C. trachomatis is due, at least partially, to the IL-10 induced during C. trachomatis infection.

Original languageEnglish
Pages (from-to)44-47
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume24
Issue number1
StatePublished - Jan 2004
Externally publishedYes

Keywords

  • Chlamydia trachomatis
  • HeLa cell
  • IL-10 antibody
  • Major histocompatibility complex class I and II molecules

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