In this study, anaerobic treatability and biogas generation potential of broiler and cattle manure were investigated. For this purpose, seven sets of anaerobic batch reactor experiments were performed using broiler and cattle manure and their mixtures in five different ratios (100% broiler; 75% broiler, 25% cattle; 50% broiler, 50% cattle; 25% broiler, 75% cattle; 100% cattle). These manure mixtures had two different initial chemical oxygen demand (COD) (12,000 and 53,500 mg/l) concentrations. The effects of initial COD concentration, nutrient and trace metal supplementation, microbial acclimation and digestion temperature were investigated. Results revealed that the efficiency of total COD removal was 32.0-43.3% and 37.9-50% for initial COD concentrations of 12,000 and 53,500 mg/l, respectively. The biogas yields observed for initial COD concentrations of 12,000 and 53,500 mg/l were 180-270 and 223-368 mlgas/g COD added, respectively. A decrease in biogas yield was observed as the fraction of broiler manure increased in mixture of broiler and cattle manure at initial COD values of 53,500 mg/l.