TY - JOUR
T1 - Elevated transforming growth factor-β concentration correlates with posttrauma immunosuppression
AU - Meert, Kathleen L.
AU - Ofenstein, John P.
AU - Genyea, Carol
AU - Sarnaik, Ashok P.
AU - Kaplan, Joseph
PY - 1996/6
Y1 - 1996/6
N2 - Objective: To determine whether trauma induces an increase in the concentration of circulating transforming growth factor-β (TGF-β), and whether there is a temporal correlation between plasma TGF-β concentration and the development of post-trauma cellular immunosuppression. Materials and Methods: Male Sprague-Dawley rats were anesthetized, subjected to bilateral femur fractures or sham injury, and killed 1, 3, or 5 days later. Plasma TGF- β levels, splenocyte phenotypes, mitogen-induced proliferation, interleukin- 2 (IL-2) production, and IL-2 receptor (IL-2R) expression were determined at each time point. Measurements and Main Results: Splenocyte proliferation increased on day 1 postinjury without corresponding change in IL-2 or plasma TGF-β levels. Splenocyte proliferation and IL-2 production were suppressed on day 3 postinjury, while plasma TGF-β levels peaked. No differences were observed between trauma and control groups on day 5. Splenocyte phenotypes and IL-2R expression were similar in injured and control rats at all times. Conclusions: Suppression of lymphocyte proliferation and IL-2 production after trauma occurs concomitantly with a rise in plasma TGF-β. The immune response is restored with normalization of TGF-β concentration. These observations suggest that TGF-β may contribute to posttrauma immunosuppression.
AB - Objective: To determine whether trauma induces an increase in the concentration of circulating transforming growth factor-β (TGF-β), and whether there is a temporal correlation between plasma TGF-β concentration and the development of post-trauma cellular immunosuppression. Materials and Methods: Male Sprague-Dawley rats were anesthetized, subjected to bilateral femur fractures or sham injury, and killed 1, 3, or 5 days later. Plasma TGF- β levels, splenocyte phenotypes, mitogen-induced proliferation, interleukin- 2 (IL-2) production, and IL-2 receptor (IL-2R) expression were determined at each time point. Measurements and Main Results: Splenocyte proliferation increased on day 1 postinjury without corresponding change in IL-2 or plasma TGF-β levels. Splenocyte proliferation and IL-2 production were suppressed on day 3 postinjury, while plasma TGF-β levels peaked. No differences were observed between trauma and control groups on day 5. Splenocyte phenotypes and IL-2R expression were similar in injured and control rats at all times. Conclusions: Suppression of lymphocyte proliferation and IL-2 production after trauma occurs concomitantly with a rise in plasma TGF-β. The immune response is restored with normalization of TGF-β concentration. These observations suggest that TGF-β may contribute to posttrauma immunosuppression.
KW - Immunosuppression
KW - Interleukin-2
KW - Lymphocytes
KW - Transforming growth factor- β
KW - Trauma
UR - http://www.scopus.com/inward/record.url?scp=0029894853&partnerID=8YFLogxK
U2 - 10.1097/00005373-199606000-00007
DO - 10.1097/00005373-199606000-00007
M3 - Article
C2 - 8656475
AN - SCOPUS:0029894853
VL - 40
SP - 901
EP - 906
JO - Journal of Trauma - Injury, Infection and Critical Care
JF - Journal of Trauma - Injury, Infection and Critical Care
SN - 1079-6061
IS - 6
ER -