Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells

Megan N. Freitas; Andrew D. Marten; Gavin A. Moore; Maya O. Tree; Sean P. McBrayer; Michael J. Conway

doi:10.1016/j.virol.2019.12.010

Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells

Megan N. Freitas, Andrew D. Marten, Gavin A. Moore, Maya O. Tree, Sean P. McBrayer, Michael J. Conway

COLLEGE OF MEDICINE

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Aedes aegypti is the primary vector of dengue virus (DENV), and acquires this virus from a vertebrate host during blood feeding. Previous literature has shown that vertebrate blood factors such as complement protein C5a and low-density lipoprotein (LDL) influence DENV acquisition in the mosquito. Here, we show that extracellular vesicles in cell culture medium inhibit DENV infection in mosquito cells. Specifically, extracellular vesicles enter into mosquito cells and inhibit an early stage of infection. Extracellular vesicles had no effect on virus cell attachment or entry. Instead, extracellular vesicles restricted virus membrane fusion. Extracellular vesicles only inhibited DENV infection in mosquito cells and not vertebrate cells. These data highlight a novel virus-vector-host interaction that limits virus infection in mosquito cells by restricting virus membrane fusion.

Original language	English
Pages (from-to)	141-149
Number of pages	9
Journal	Virology
Volume	541
DOIs	https://doi.org/10.1016/j.virol.2019.12.010
State	Published - Feb 2020

Keywords

Dengue
Exosome
Extracellular vesicle
Fusion
Lipid
Low-density lipoprotein
Mosquito

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2019.12.010

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@article{9ca906ffda3c4f97b9ea19edc66138db,

title = "Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells",

abstract = "Aedes aegypti is the primary vector of dengue virus (DENV), and acquires this virus from a vertebrate host during blood feeding. Previous literature has shown that vertebrate blood factors such as complement protein C5a and low-density lipoprotein (LDL) influence DENV acquisition in the mosquito. Here, we show that extracellular vesicles in cell culture medium inhibit DENV infection in mosquito cells. Specifically, extracellular vesicles enter into mosquito cells and inhibit an early stage of infection. Extracellular vesicles had no effect on virus cell attachment or entry. Instead, extracellular vesicles restricted virus membrane fusion. Extracellular vesicles only inhibited DENV infection in mosquito cells and not vertebrate cells. These data highlight a novel virus-vector-host interaction that limits virus infection in mosquito cells by restricting virus membrane fusion.",

keywords = "Dengue, Exosome, Extracellular vesicle, Fusion, Lipid, Low-density lipoprotein, Mosquito",

author = "Freitas, {Megan N.} and Marten, {Andrew D.} and Moore, {Gavin A.} and Tree, {Maya O.} and McBrayer, {Sean P.} and Conway, {Michael J.}",

note = "Funding Information: This work was supported through start-up funds from Central Michigan University College of Medicine. Funding Information: This work was supported through start-up funds from Central Michigan University College of Medicine. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2020",

month = feb,

doi = "10.1016/j.virol.2019.12.010",

language = "English",

volume = "541",

pages = "141--149",

journal = "Virology",

issn = "0042-6822",

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T1 - Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells

AU - Freitas, Megan N.

AU - Marten, Andrew D.

AU - Moore, Gavin A.

AU - Tree, Maya O.

AU - McBrayer, Sean P.

AU - Conway, Michael J.

N1 - Funding Information: This work was supported through start-up funds from Central Michigan University College of Medicine. Funding Information: This work was supported through start-up funds from Central Michigan University College of Medicine. Publisher Copyright: © 2019 Elsevier Inc.

PY - 2020/2

Y1 - 2020/2

N2 - Aedes aegypti is the primary vector of dengue virus (DENV), and acquires this virus from a vertebrate host during blood feeding. Previous literature has shown that vertebrate blood factors such as complement protein C5a and low-density lipoprotein (LDL) influence DENV acquisition in the mosquito. Here, we show that extracellular vesicles in cell culture medium inhibit DENV infection in mosquito cells. Specifically, extracellular vesicles enter into mosquito cells and inhibit an early stage of infection. Extracellular vesicles had no effect on virus cell attachment or entry. Instead, extracellular vesicles restricted virus membrane fusion. Extracellular vesicles only inhibited DENV infection in mosquito cells and not vertebrate cells. These data highlight a novel virus-vector-host interaction that limits virus infection in mosquito cells by restricting virus membrane fusion.

AB - Aedes aegypti is the primary vector of dengue virus (DENV), and acquires this virus from a vertebrate host during blood feeding. Previous literature has shown that vertebrate blood factors such as complement protein C5a and low-density lipoprotein (LDL) influence DENV acquisition in the mosquito. Here, we show that extracellular vesicles in cell culture medium inhibit DENV infection in mosquito cells. Specifically, extracellular vesicles enter into mosquito cells and inhibit an early stage of infection. Extracellular vesicles had no effect on virus cell attachment or entry. Instead, extracellular vesicles restricted virus membrane fusion. Extracellular vesicles only inhibited DENV infection in mosquito cells and not vertebrate cells. These data highlight a novel virus-vector-host interaction that limits virus infection in mosquito cells by restricting virus membrane fusion.

KW - Dengue

KW - Exosome

KW - Extracellular vesicle

KW - Fusion

KW - Lipid

KW - Low-density lipoprotein

KW - Mosquito

UR - http://www.scopus.com/inward/record.url?scp=85077087250&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2019.12.010

DO - 10.1016/j.virol.2019.12.010

M3 - Article

C2 - 32056712

AN - SCOPUS:85077087250

SN - 0042-6822

VL - 541

SP - 141

EP - 149

JO - Virology

JF - Virology

ER -

Extracellular vesicles restrict dengue virus fusion in Aedes aegypti cells

Abstract

Keywords

UN SDGs

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