Abstract
The ability to place a series of gene constructs at a specific site in the genome opens new possibilities for the experimental examination of gene expression and chromosomal position effects. We report that the FLP-FRT site-specific recombination system of the yeast 2μ plasmid can be used to integrate DNA at a chromosomal FRT target site in Drosophila. The technique we used was to first integrate an FRT-flanked gene by standard P element-mediated transformation. FLP was then used to excise the FRT-flanked donor DNA and screen for FLP-mediated re-integration at an FRT target at a different chromosome location. Such events were recovered from up to 5% of the crosses used to screen for mobilization and are easily detectable by altered linkage of a white reporter gene or by the generation of a white+ gene upon integration.
Original language | English |
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Pages (from-to) | 3665-3671 |
Number of pages | 7 |
Journal | Nucleic Acids Research |
Volume | 25 |
Issue number | 18 |
DOIs | |
State | Published - Sep 15 1997 |