Fluorometric assay for detection and quantitation of polyamidoamine dendrimers

A. Sharma, M. Rao, R. Miller, A. Desai

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Polyamidoamine dendrimers are protein-like nanomaterials that have many potential biological applications. Research studies using dendrimers often require their detection and quantitation. This article describes a simple, inexpensive, and rapid fluorometric assay based on the ability of dendrimers to bind the fluorescent probe, 8-anilino-1-naphthalene-sulfonic acid. This interaction leads to an enhanced fluorescence intensity of the dye that shows a linear relationship with dendrimer concentration. The procedure is applicable to dendrimers that have various surface functional groups and internal cores. Amine- and hydroxyl-terminated dendrimers gave the best calibration curves under alkaline conditions (pH 7.4). Carboxyl-terminated dendrimers required acidic conditions for their measurement. The assay for dendrimers with an amine surface and an ethylene diamine core was accurate between 0 and 100 μM and produced a within-run coefficient of less than 5%. Under the assay conditions used, the fluorescence signal strength was dependent on type of surface group and core length of the dendrimer.

Original languageEnglish
Pages (from-to)70-75
Number of pages6
JournalAnalytical Biochemistry
Issue number1
StatePublished - Sep 1 2005


  • 8-Anilino-1- naphthalene-sulfonic acid
  • Fluorescence spectroscopy
  • Polyamidoamine dendrimers


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