@article{488dcff1b6ea40a481c4af44048a06b5,
title = "Freshwater mussels and host fish gut microbe community composition shifts after agricultural contaminant exposure",
abstract = "Aims: We examined the effects of a mixture of contaminants found in agricultural watersheds on the gut microbiota and physiology of both the freshwater mussel Lampsilis cardium, and L. cardium host fish Micropterus salmoides. Methods and results: Lampsilis cardium and M. salmoides were exposed to three concentrations of agricultural contaminants for 60 days (observing behaviour daily) before being sampled for gut microbiota analyses. DNA was extracted from the gut samples, amplified via PCR, and sequenced using the Illumina Mi-Seq platform. Only L. cardium guts had differing microbiota across treatments, with an increase in potentially pathogenic Aeromonas. We also provide novel evidence of a core microbiota within L. cardium and M. salmoides. In terms of physiology, female L. cardium exhibited a decrease in movement and marsupial gill display in contaminant exposures. Conclusions: Exposure to contaminants from agricultural watersheds may affect population recruitment within freshwater mussel communities over time. Specifically, increased pathogenic micro-organisms and altered behaviour can reduce the likelihood of glochidia dispersal. Significance and impact of the study: This study supports emerging research that contaminants found in agricultural watersheds may be a factor in freshwater mussel population declines. It also provides novel evidence that unionids have a core gut microbiota.",
keywords = "behaviour, contaminants, freshwater mussel, host fish, metagenomics, sequencing",
author = "Gill, {Stephanie P.} and Learman, {Deric R.} and Annis, {Mandy L.} and Woolnough, {Daelyn A.}",
note = "Funding Information: We acknowledge the immense support of the U.S. Fish and Wildlife Service, particularly that of Stephanie Hummel without whom the project would not have been possible. Central Michigan University (CMU) Woolnough Lab students Rachel Paull and Megan Malish helped running PCRs. Analyses using Mothur were performed with code from Dean Horton (Cleveland Clinic Genomics Core). Jessica Zehnpfennig (CMU) deserves acknowledgement for donating her time to push this project through the HPCC system. Analyses on organized sequences were performed through the HPCC system at Michigan State University. Next-generation sequencing was performed with the help of the Michigan State University's Research Technology Support Facility. Feedback from Dr. Greg Cope (University of North Carolina) during the early stages of this study and manuscript development was greatly appreciated. We thank anonymous reviewers as well as editors that have helped improve this manuscript. Funding was provided from the Great Lakes Restoration Initiative through the U.S. Fish and Wildlife Service's Contaminants of Emerging Concern Team and Central Michigan University. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service or the U.S. Environmental Protection Agency. Fish collection and laboratory experiments followed Central Michigan University protocols and were approved by the Institutional Animal Care and Use Committee (IACUC); IACUC approval number 17-11. Unionid collection was completed under Michigan Cultural or Scientific Collection and Threatened and Endangered Species Permit (2017–2018) as well as US Fish & Wildlife Services Federal Endangered Species Permit (TE71821A-3). This paper is contribution # 173 of the CMU Institute for Great Lakes Research. Funding Information: We acknowledge the immense support of the U.S. Fish and Wildlife Service, particularly that of Stephanie Hummel without whom the project would not have been possible. Central Michigan University (CMU) Woolnough Lab students Rachel Paull and Megan Malish helped running PCRs. Analyses using Mothur were performed with code from Dean Horton (Cleveland Clinic Genomics Core). Jessica Zehnpfennig (CMU) deserves acknowledgement for donating her time to push this project through the HPCC system. Analyses on organized sequences were performed through the HPCC system at Michigan State University. Next‐generation sequencing was performed with the help of the Michigan State University's Research Technology Support Facility. Feedback from Dr. Greg Cope (University of North Carolina) during the early stages of this study and manuscript development was greatly appreciated. We thank anonymous reviewers as well as editors that have helped improve this manuscript. Funding was provided from the Great Lakes Restoration Initiative through the U.S. Fish and Wildlife Service's Contaminants of Emerging Concern Team and Central Michigan University. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service or the U.S. Environmental Protection Agency. Fish collection and laboratory experiments followed Central Michigan University protocols and were approved by the Institutional Animal Care and Use Committee (IACUC); IACUC approval number 17‐11. Unionid collection was completed under Michigan Cultural or Scientific Collection and Threatened and Endangered Species Permit (2017–2018) as well as US Fish & Wildlife Services Federal Endangered Species Permit (TE71821A‐3). This paper is contribution # 173 of the CMU Institute for Great Lakes Research. Publisher Copyright: {\textcopyright} 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.",
year = "2022",
month = dec,
doi = "10.1111/jam.15801",
language = "English",
volume = "133",
pages = "3645--3658",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Journal of Applied Microbiology",
number = "6",
}