Abstract
Objective: To explore the genetic polymorphism of HLA-Cw locus in Southern Chinese Han Population(SCHP). Methods: HLA-Cw loci were genotyped for a panel of sixty randomly selected healthy SCHP individuals, using the ARMS/PCR technology described in 12th IHWC. Results: Twelve HLA-Cw alleles or allele-groups were detected with HLA-Cw * 07, * 01, * 03 as the most common HLA-Cw genes. HLA-Cw * 08, previously thought to be rare in SCHP by serological typing, occupied a gene frequency of 0.1056. In addition, four kinds of non-serologically defined HLA-Cw * genes i.e. HLA-Cw * 1202, * 1203, * 14, and * 15 were found in the target population sharing 0.0673 of the total HLA-Cw frequency. Conclusion: Normal HLA-Cw gene frequencies of SCHP were identified in the present study; The results also confirmed that ARMS/PCR was a reliable and fast method for HLA-Cw genotyping.
| Original language | English |
|---|---|
| Pages (from-to) | 95-98 |
| Number of pages | 4 |
| Journal | Chinese Journal of Microbiology and Immunology |
| Volume | 22 |
| Issue number | 1 |
| State | Published - Jan 2002 |
| Externally published | Yes |
Keywords
- HLA-Cw
- Polymerase chain reaction
- Sequence specific primer