Objective: To explore the genetic polymorphism of HLA-Cw locus in Southern Chinese Han Population(SCHP). Methods: HLA-Cw loci were genotyped for a panel of sixty randomly selected healthy SCHP individuals, using the ARMS/PCR technology described in 12th IHWC. Results: Twelve HLA-Cw alleles or allele-groups were detected with HLA-Cw * 07, * 01, * 03 as the most common HLA-Cw genes. HLA-Cw * 08, previously thought to be rare in SCHP by serological typing, occupied a gene frequency of 0.1056. In addition, four kinds of non-serologically defined HLA-Cw * genes i.e. HLA-Cw * 1202, * 1203, * 14, and * 15 were found in the target population sharing 0.0673 of the total HLA-Cw frequency. Conclusion: Normal HLA-Cw gene frequencies of SCHP were identified in the present study; The results also confirmed that ARMS/PCR was a reliable and fast method for HLA-Cw genotyping.
|Number of pages||4|
|Journal||Chinese Journal of Microbiology and Immunology|
|State||Published - Jan 2002|
- Polymerase chain reaction
- Sequence specific primer