Greater filamin C, GSK3α, and GSK3β serine phosphorylation in insulin-stimulated isolated skeletal muscles of calorie restricted 24 month-old rats

Donel A. Sequea, Naveen Sharma, Edward B. Arias, Gregory D. Cartee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence on site-specific phosphorylation of five Akt substrates (AS160Ser588, TBC1D1Thr596, FLNcSer2213, GSK3αSer21, and GSK3βSer9) in predominantly fast-twitch (epitrochlearis) and predominantly slow-twitch (soleus) muscles. We observed no CR-effect on phosphorylation of AS160Ser588 or TBC1D1Thr596, but there was a CR-induced increase in insulin-stimulated FLNcSer2213, GSK3αSer21, and GSK3βSer9 phosphorylation for both muscles. These results indicate that CR does not uniformly affect insulin-mediated phosphorylation of Akt substrates in fast- or slow-twitch muscles from 24 month-old rats.

Original languageEnglish
Pages (from-to)60-63
Number of pages4
JournalMechanisms of Ageing and Development
Volume134
Issue number1-2
DOIs
StatePublished - Jan 2013

Keywords

  • Ageing
  • Dietary restriction
  • Insulin signaling
  • Muscle fiber type

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