Guidelines for experimental models of myocardial ischemia and infarction

Merry L. Lindsey; Roberto Bolli; John M. Canty; Xiao Jun Du; Nikolaos G. Frangogiannis; Stefan Frantz; Robert G. Gourdie; Jeffrey W. Holmes; Steven P. Jones; Robert A. Kloner; David J. Lefer; Ronglih Liao; Elizabeth Murphy; Peipei Ping; Karin Przyklenk; Fabio A. Recchia; Lisa Schwartz Longacre; Crystal M. Ripplinger; Jennifer E. Van Eyk; Gerd Heusch

doi:10.1152/ajpheart.00335.2017

Guidelines for experimental models of myocardial ischemia and infarction

Merry L. Lindsey, Roberto Bolli, John M. Canty, Xiao Jun Du, Nikolaos G. Frangogiannis, Stefan Frantz, Robert G. Gourdie, Jeffrey W. Holmes, Steven P. Jones, Robert A. Kloner, David J. Lefer, Ronglih Liao, Elizabeth Murphy, Peipei Ping, Karin Przyklenk, Fabio A. Recchia, Lisa Schwartz Longacre, Crystal M. Ripplinger, Jennifer E. Van Eyk, Gerd Heusch

Research output: Contribution to journal › Review article › peer-review

320 Scopus citations

Abstract

Myocardial infarction is a prevalent major cardiovascular event that arises from myocardial ischemia with or without reperfusion, and basic and translational research is needed to better understand its underlying mechanisms and consequences for cardiac structure and function. Ischemia underlies a broad range of clinical scenarios ranging from angina to hibernation to permanent occlusion, and while reperfusion is mandatory for salvage from ischemic injury, reperfusion also inflicts injury on its own. In this consensus statement, we present recommendations for animal models of myocardial ischemia and infarction. With increasing awareness of the need for rigor and reproducibility in designing and performing scientific research to ensure validation of results, the goal of this review is to provide best practice information regarding myocardial ischemia-reperfusion and infarction models.

Original language	English
Pages (from-to)	H812-H838
Journal	American Journal of Physiology - Heart and Circulatory Physiology
Volume	314
Issue number	4
DOIs	https://doi.org/10.1152/ajpheart.00335.2017
State	Published - Apr 2018

Keywords

Animal models
Cardiac remodeling
Heart failure
Myocardial infarction
Reperfusion
Rigor and reproducibility

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10.1152/ajpheart.00335.2017

Cite this

Lindsey, M. L., Bolli, R., Canty, J. M., Du, X. J., Frangogiannis, N. G., Frantz, S., Gourdie, R. G., Holmes, J. W., Jones, S. P., Kloner, R. A., Lefer, D. J., Liao, R., Murphy, E., Ping, P., Przyklenk, K., Recchia, F. A., Longacre, L. S., Ripplinger, C. M., Van Eyk, J. E., & Heusch, G. (2018). Guidelines for experimental models of myocardial ischemia and infarction. American Journal of Physiology - Heart and Circulatory Physiology, 314(4), H812-H838. https://doi.org/10.1152/ajpheart.00335.2017

@article{4a9f8e00daef4a7a89dc0c9db2e29571,

title = "Guidelines for experimental models of myocardial ischemia and infarction",

abstract = "Myocardial infarction is a prevalent major cardiovascular event that arises from myocardial ischemia with or without reperfusion, and basic and translational research is needed to better understand its underlying mechanisms and consequences for cardiac structure and function. Ischemia underlies a broad range of clinical scenarios ranging from angina to hibernation to permanent occlusion, and while reperfusion is mandatory for salvage from ischemic injury, reperfusion also inflicts injury on its own. In this consensus statement, we present recommendations for animal models of myocardial ischemia and infarction. With increasing awareness of the need for rigor and reproducibility in designing and performing scientific research to ensure validation of results, the goal of this review is to provide best practice information regarding myocardial ischemia-reperfusion and infarction models.",

keywords = "Animal models, Cardiac remodeling, Heart failure, Myocardial infarction, Reperfusion, Rigor and reproducibility",

author = "Lindsey, {Merry L.} and Roberto Bolli and Canty, {John M.} and Du, {Xiao Jun} and Frangogiannis, {Nikolaos G.} and Stefan Frantz and Gourdie, {Robert G.} and Holmes, {Jeffrey W.} and Jones, {Steven P.} and Kloner, {Robert A.} and Lefer, {David J.} and Ronglih Liao and Elizabeth Murphy and Peipei Ping and Karin Przyklenk and Recchia, {Fabio A.} and Longacre, {Lisa Schwartz} and Ripplinger, {Crystal M.} and {Van Eyk}, {Jennifer E.} and Gerd Heusch",

note = "Funding Information: 1Mississippi Center for Heart Research, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; 2Research Service, G. V. (Sonny) Montgomery Veterans Affairs Medical Center, Jackson, Mississippi; 3Division of Cardiovascular Medicine and Institute of Molecular Cardiology, University of Louisville, Louisville, Kentucky; 4Division of Cardiovascular Medicine, Departments of Biomedical Engineering and Physiology and Biophysics, The Veterans Affairs Western New York Health Care System and Clinical and Translational Science Institute, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York; 5Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia; 6The Wilf Family Cardiovascular Research Institute, Department of Medicine (Cardiology), Albert Einstein College of Medicine, Bronx, New York; 7Department of Internal Medicine I, University Hospital, W{\"u}rzburg, Germany; 8Center for Heart and Regenerative Medicine Research, Virginia Tech Carilion Research Institute, Roanoke, Virginia; 9Department of Biomedical Engineering, University of Virginia Health System, Charlottesville, Virginia; 10Department of Medicine, Institute of Molecular Cardiology, Diabetes and Obesity Center, University of Louisville, Louisville, Kentucky; 11HMRI Cardiovascular Research Institute, Huntington Medical Research Institutes, Pasadena, California; 12Division of Cardiovascular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California; 13Cardiovascular Center of Excellence, Louisiana State University Health Science Center, New Orleans, Louisiana; 14Harvard Medical School, Boston, Massachusetts; 15Division of Genetics and Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women{\textquoteright}s Hospital, Boston, Massachusetts; 16Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland; 17National Institutes of Health BD2KBig Data to Knowledge (BD2K) Center of Excellence and Department of Physiology, Medicine and Bioinformatics, University of California, Los Angeles, California; 18Cardiovascular Research Institute and Departments of Physiology and Emergency Medicine, Wayne State University School of Medicine, Detroit, Michigan; 19Institute of Life Sciences, Scuola Superiore Sant{\textquoteright}Anna, Fondazione G. Monasterio, Pisa, Italy; 20Cardiovascular Research Center, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania; 21Heart Failure and Arrhythmias Branch, Division of Cardiovascular Sciences, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland; 22Department of Pharmacology, School of Medicine, University of California, Davis, California; 23The Smidt Heart Institute, Department of Medicine, Cedars Sinai Medical Center, Los Angeles, California; and 24Institute for Pathophysiology, West German Heart and Vascular Center, University of Essen Medical School, Essen, Germany Funding Information: We acknowledge support from the following: National Institutes of Health Grants HL-002066, HL-051971, HL-056728, HL-061610, HL- 075360, HL-078825, HL-088533, HL-092141, HL-093579, HL-107153, HL-111600, HL-112730, HL-112831, HL-113452, HL-113530, HL-116449, HL-128135, HL-129120, HL-129823, HL-130266, HL-131647, HL-132075, HL-135772, GM-103492, HL-131647, HL-76246, HL-85440, GM-104357, GM-114833, GM-115428, and UL1-TR-001412; American Heart Association Grants 16GRNT30960054 and 16CSA28880004; Grand Challenge Award; Department of Defense Grants 16W81XWH-16–1-0592, PR151051, PR151134, and PR151029; Biomedical Laboratory Research and Development Service of the Veterans Affairs Office of Research and Development Awards 1IO1BX002659 and I01BX000505; Australian National Health and Medical Research Council Research Fellowship APP1043026; Bundesministerium f{\"u}r Bildung und Forschung Grant BMBF01 EO1004; and German Research Foundation Grants DFG He 1320/18-3 and SFB 1116 B8. R. A. Kloner reports grant support from Stealth Biotherapeutics, Servier, Inc., and Faraday to test experimental compounds in experimental myocardial infarction models. Funding Information: The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, American Heart Association, United States Department of Defense, United States Veterans Administration, National Health and Medical Research Council, or German Research Foundation. Funding Information: We acknowledge support from the following: National Institutes of Health Grants HL-002066, HL-051971, HL-056728, HL-061610, HL-075360, HL-078825, HL-088533, HL-092141, HL-093579, HL-107153, HL-111600, HL-112730, HL-112831, HL-113452, HL-113530, HL-116449, HL-128135, HL-129120, HL-129823, HL-130266, HL-131647, HL-132075, HL-135772, GM-103492, HL-131647, HL-76246, HL-85440, GM-104357, GM-114833, GM-115428, and UL1-TR-001412; American Heart Association Grants 16GRNT30960054 and 16CSA28880004; Grand Challenge Award; Department of Defense Grants 16W81XWH-16–1-0592, PR151051, PR151134, and PR151029; Biomedical Laboratory Research and Development Service of the Veterans Affairs Office of Research and Development Awards 1IO1BX002659 and I01BX000505; Australian National Health and Medical Research Council Research Fellowship APP1043026; Bundesministerium f{\"u}r Bildung und Forschung Grant BMBF01 EO1004; and German Research Foundation Grants DFG He 1320/18-3 and SFB 1116 B8. R. A. Kloner reports grant support from Stealth Biotherapeutics, Servier, Inc., and Faraday to test experimental compounds in experimental myocardial infarction models. Funding Information: Much of the lack of reproducibility has been ascribed to limited or lacking scientific rigor (29, 250). In response to these and other concerns, the United States National Institutes of Health now includes explicit requirements for applicants to show, and reviewers to evaluate, the level of scientific rigor in grant applications. Whether suboptimal rigor fully explains and underlies the reproducibility crisis is unclear (162). Nonetheless, advocating for reproducibility and scientific rigor is welcome. Publisher Copyright: {\textcopyright} 2018 American Physiological Society. All rights reserved.",

year = "2018",

month = apr,

doi = "10.1152/ajpheart.00335.2017",

language = "English",

volume = "314",

pages = "H812--H838",

journal = "American Journal of Physiology - Heart and Circulatory Physiology",

issn = "0363-6135",

number = "4",

}

Lindsey, ML, Bolli, R, Canty, JM, Du, XJ, Frangogiannis, NG, Frantz, S, Gourdie, RG, Holmes, JW, Jones, SP, Kloner, RA, Lefer, DJ, Liao, R, Murphy, E, Ping, P, Przyklenk, K, Recchia, FA, Longacre, LS, Ripplinger, CM, Van Eyk, JE & Heusch, G 2018, 'Guidelines for experimental models of myocardial ischemia and infarction', American Journal of Physiology - Heart and Circulatory Physiology, vol. 314, no. 4, pp. H812-H838. https://doi.org/10.1152/ajpheart.00335.2017

TY - JOUR

T1 - Guidelines for experimental models of myocardial ischemia and infarction

AU - Lindsey, Merry L.

AU - Bolli, Roberto

AU - Canty, John M.

AU - Du, Xiao Jun

AU - Frangogiannis, Nikolaos G.

AU - Frantz, Stefan

AU - Gourdie, Robert G.

AU - Holmes, Jeffrey W.

AU - Jones, Steven P.

AU - Kloner, Robert A.

AU - Lefer, David J.

AU - Liao, Ronglih

AU - Murphy, Elizabeth

AU - Ping, Peipei

AU - Przyklenk, Karin

AU - Recchia, Fabio A.

AU - Longacre, Lisa Schwartz

AU - Ripplinger, Crystal M.

AU - Van Eyk, Jennifer E.

AU - Heusch, Gerd

N1 - Funding Information: 1Mississippi Center for Heart Research, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; 2Research Service, G. V. (Sonny) Montgomery Veterans Affairs Medical Center, Jackson, Mississippi; 3Division of Cardiovascular Medicine and Institute of Molecular Cardiology, University of Louisville, Louisville, Kentucky; 4Division of Cardiovascular Medicine, Departments of Biomedical Engineering and Physiology and Biophysics, The Veterans Affairs Western New York Health Care System and Clinical and Translational Science Institute, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York; 5Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia; 6The Wilf Family Cardiovascular Research Institute, Department of Medicine (Cardiology), Albert Einstein College of Medicine, Bronx, New York; 7Department of Internal Medicine I, University Hospital, Würzburg, Germany; 8Center for Heart and Regenerative Medicine Research, Virginia Tech Carilion Research Institute, Roanoke, Virginia; 9Department of Biomedical Engineering, University of Virginia Health System, Charlottesville, Virginia; 10Department of Medicine, Institute of Molecular Cardiology, Diabetes and Obesity Center, University of Louisville, Louisville, Kentucky; 11HMRI Cardiovascular Research Institute, Huntington Medical Research Institutes, Pasadena, California; 12Division of Cardiovascular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California; 13Cardiovascular Center of Excellence, Louisiana State University Health Science Center, New Orleans, Louisiana; 14Harvard Medical School, Boston, Massachusetts; 15Division of Genetics and Division of Cardiovascular Medicine, Department of Medicine, Brigham and Women’s Hospital, Boston, Massachusetts; 16Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland; 17National Institutes of Health BD2KBig Data to Knowledge (BD2K) Center of Excellence and Department of Physiology, Medicine and Bioinformatics, University of California, Los Angeles, California; 18Cardiovascular Research Institute and Departments of Physiology and Emergency Medicine, Wayne State University School of Medicine, Detroit, Michigan; 19Institute of Life Sciences, Scuola Superiore Sant’Anna, Fondazione G. Monasterio, Pisa, Italy; 20Cardiovascular Research Center, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania; 21Heart Failure and Arrhythmias Branch, Division of Cardiovascular Sciences, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland; 22Department of Pharmacology, School of Medicine, University of California, Davis, California; 23The Smidt Heart Institute, Department of Medicine, Cedars Sinai Medical Center, Los Angeles, California; and 24Institute for Pathophysiology, West German Heart and Vascular Center, University of Essen Medical School, Essen, Germany Funding Information: We acknowledge support from the following: National Institutes of Health Grants HL-002066, HL-051971, HL-056728, HL-061610, HL- 075360, HL-078825, HL-088533, HL-092141, HL-093579, HL-107153, HL-111600, HL-112730, HL-112831, HL-113452, HL-113530, HL-116449, HL-128135, HL-129120, HL-129823, HL-130266, HL-131647, HL-132075, HL-135772, GM-103492, HL-131647, HL-76246, HL-85440, GM-104357, GM-114833, GM-115428, and UL1-TR-001412; American Heart Association Grants 16GRNT30960054 and 16CSA28880004; Grand Challenge Award; Department of Defense Grants 16W81XWH-16–1-0592, PR151051, PR151134, and PR151029; Biomedical Laboratory Research and Development Service of the Veterans Affairs Office of Research and Development Awards 1IO1BX002659 and I01BX000505; Australian National Health and Medical Research Council Research Fellowship APP1043026; Bundesministerium für Bildung und Forschung Grant BMBF01 EO1004; and German Research Foundation Grants DFG He 1320/18-3 and SFB 1116 B8. R. A. Kloner reports grant support from Stealth Biotherapeutics, Servier, Inc., and Faraday to test experimental compounds in experimental myocardial infarction models. Funding Information: The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health, American Heart Association, United States Department of Defense, United States Veterans Administration, National Health and Medical Research Council, or German Research Foundation. Funding Information: We acknowledge support from the following: National Institutes of Health Grants HL-002066, HL-051971, HL-056728, HL-061610, HL-075360, HL-078825, HL-088533, HL-092141, HL-093579, HL-107153, HL-111600, HL-112730, HL-112831, HL-113452, HL-113530, HL-116449, HL-128135, HL-129120, HL-129823, HL-130266, HL-131647, HL-132075, HL-135772, GM-103492, HL-131647, HL-76246, HL-85440, GM-104357, GM-114833, GM-115428, and UL1-TR-001412; American Heart Association Grants 16GRNT30960054 and 16CSA28880004; Grand Challenge Award; Department of Defense Grants 16W81XWH-16–1-0592, PR151051, PR151134, and PR151029; Biomedical Laboratory Research and Development Service of the Veterans Affairs Office of Research and Development Awards 1IO1BX002659 and I01BX000505; Australian National Health and Medical Research Council Research Fellowship APP1043026; Bundesministerium für Bildung und Forschung Grant BMBF01 EO1004; and German Research Foundation Grants DFG He 1320/18-3 and SFB 1116 B8. R. A. Kloner reports grant support from Stealth Biotherapeutics, Servier, Inc., and Faraday to test experimental compounds in experimental myocardial infarction models. Funding Information: Much of the lack of reproducibility has been ascribed to limited or lacking scientific rigor (29, 250). In response to these and other concerns, the United States National Institutes of Health now includes explicit requirements for applicants to show, and reviewers to evaluate, the level of scientific rigor in grant applications. Whether suboptimal rigor fully explains and underlies the reproducibility crisis is unclear (162). Nonetheless, advocating for reproducibility and scientific rigor is welcome. Publisher Copyright: © 2018 American Physiological Society. All rights reserved.

PY - 2018/4

Y1 - 2018/4

N2 - Myocardial infarction is a prevalent major cardiovascular event that arises from myocardial ischemia with or without reperfusion, and basic and translational research is needed to better understand its underlying mechanisms and consequences for cardiac structure and function. Ischemia underlies a broad range of clinical scenarios ranging from angina to hibernation to permanent occlusion, and while reperfusion is mandatory for salvage from ischemic injury, reperfusion also inflicts injury on its own. In this consensus statement, we present recommendations for animal models of myocardial ischemia and infarction. With increasing awareness of the need for rigor and reproducibility in designing and performing scientific research to ensure validation of results, the goal of this review is to provide best practice information regarding myocardial ischemia-reperfusion and infarction models.

AB - Myocardial infarction is a prevalent major cardiovascular event that arises from myocardial ischemia with or without reperfusion, and basic and translational research is needed to better understand its underlying mechanisms and consequences for cardiac structure and function. Ischemia underlies a broad range of clinical scenarios ranging from angina to hibernation to permanent occlusion, and while reperfusion is mandatory for salvage from ischemic injury, reperfusion also inflicts injury on its own. In this consensus statement, we present recommendations for animal models of myocardial ischemia and infarction. With increasing awareness of the need for rigor and reproducibility in designing and performing scientific research to ensure validation of results, the goal of this review is to provide best practice information regarding myocardial ischemia-reperfusion and infarction models.

KW - Animal models

KW - Cardiac remodeling

KW - Heart failure

KW - Myocardial infarction

KW - Reperfusion

KW - Rigor and reproducibility

UR - http://www.scopus.com/inward/record.url?scp=85043767836&partnerID=8YFLogxK

U2 - 10.1152/ajpheart.00335.2017

DO - 10.1152/ajpheart.00335.2017

M3 - Review article

C2 - 29351451

AN - SCOPUS:85043767836

SN - 0363-6135

VL - 314

SP - H812-H838

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

IS - 4

ER -

Guidelines for experimental models of myocardial ischemia and infarction

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