Abstract
Objective: To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells. Methods: The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay. Results: The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis. The concentrations of IL-8 and IL-10 began to increase obviously at 24 h, and reached their highest level at 72 h, and then decreased. Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro. Conclusion: The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time depenedent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.
| Original language | English |
|---|---|
| Pages (from-to) | 174-176 |
| Number of pages | 3 |
| Journal | Bulletin of Hunan Medical University |
| Volume | 28 |
| Issue number | 2 |
| State | Published - Apr 28 2003 |
| Externally published | Yes |
Keywords
- Chlamydia trachomatis
- Hela cell
- Interleukin-10
- Interleukin-8