Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia trachomatis entry

Jun long Yu, Ping Yu, Li xin Li

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Objective: To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells. Methods: The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay. Results: The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis. The concentrations of IL-8 and IL-10 began to increase obviously at 24 h, and reached their highest level at 72 h, and then decreased. Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro. Conclusion: The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time depenedent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.

Original languageEnglish
Pages (from-to)174-176
Number of pages3
JournalBulletin of Hunan Medical University
Volume28
Issue number2
StatePublished - Apr 28 2003
Externally publishedYes

Keywords

  • Chlamydia trachomatis
  • Hela cell
  • Interleukin-10
  • Interleukin-8

Fingerprint

Dive into the research topics of 'Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia trachomatis entry'. Together they form a unique fingerprint.

Cite this