@inbook{6c69d976cccd4210a44ae18dfaf738e0,
title = "Identification of Essential Genes Using Sequential CRISPR and siRNA Screens",
abstract = "Genome-wide CRISPR and siRNA screening methodologies are powerful tools that are aptly suited to the discovery of essential genes. In this chapter, we outline our methods to conduct sequential CRISPR and siRNA screens to quickly and efficiently identify essential genes within a collection of cell lines. The utilization of both screening methodologies provides a pipeline that minimizes costs and time while enabling the robust detection of candidate genes.",
keywords = "CRISPR, Essential genes, siRNA",
author = "Luke DeHart and Yockey, {Oliver P.} and Jesse Bakke",
note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1720-5_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "89--107",
booktitle = "Methods in Molecular Biology",
}