Ifet-1 is a broad-scale translational repressor required for normal P granule formation in C. elegans

Madhu S. Sengupta, Wai Yee Low, Joseph R. Patterson, Hyun Min Kim, Ana Traven, Traude H. Beilharz, Monica P. Colaiácovo, Jennifer A. Schisa, Peter R. Boag

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Large cytoplasmic ribonucleoprotein germ granule complexes are a common feature in germ cells. In C. elegans these are called P granules and for much of the life-cycle they associate with nuclear pore complexes in germ cells. P granules are rich in proteins that function in diverse RNA pathways. Here we report that the C. elegans homolog of the eIF4E-transporter IFET-1 is required for oogenesis but not spermatogenesis. We show that IFET-1 is required for translational repression of several maternal mRNAs in the distal gonad and functions in conjunction with the broad-scale translational regulators CGH-1, CAR-1 and PATR-1 to regulate germ cell sex determination. Furthermore we have found that IFET-1 localizes to P granules throughout the gonad and in the germ cell lineage in the embryo. Interestingly, IFET-1 is required for the normal ultrastructure of P granules and for the localization of CGH-1 and CAR-1 to P granules. Our findings suggest that IFET-1 is a key translational regulator and is required for normal P granule formation.

Original languageEnglish
Pages (from-to)850-859
Number of pages10
JournalJournal of Cell Science
Volume126
Issue number3
DOIs
StatePublished - Feb 2013

Keywords

  • Caenorhabditis elegans
  • Eif4E transporter
  • Germ granules
  • P granule
  • Translational regulation

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