Immunity to transplantable nitrosourea-induced neurogenic tumors: II. Immune-prophylaxis of tumors of the brain

Yoshihisa Kida, Humberto Cravioto, Gerald M. Hochwald, Ute Hochgeschwender, Joseph Ransohoff

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

An effective method was sought to immunize rats against the growth of intracerebrally (IC) injected T9, tumor, a gliosarcoma cell line. Rats which were immunized with either 106 T9, cells mixed with 0.14 mg C. parvum, or 107 irradiated T9 cells showed tumor immunity to intradermal (ID) transplantation. However, to obtain tumor immunity to an IC challenge of T9 cells, rats initially had to reject an ID challenge of T9, cells. After sequential rejections of ID challenges of as many as 107 cells, a high degree of immunity was obtained, allowing rejection of up to 5 × 106 T9, cells injected IC. Spleen cells from highly immunized rats mixed with T9, cells at a ratio of 1:25 (tumor: spleen) destroyed T9, tumor cells in a Winn test. Normal spleen cells had no effect on tumor growth. We conclude that: 1) T9 cells are moderately immunogenic, 2) an effective method of immunization of CDF rats against ID transplanted T9 cells is 106 T9 cells with 0.14 mg C. parvum or 107 irradiated T9 cells, 3) a higher degree of tumor immunity is necessary to reach the brain than is needed to reach the periphery, and 4) spleen cells of highly immunized rats are cytotoxic to T9 tumor cells.

Original languageEnglish
Pages (from-to)122-135
Number of pages14
JournalJournal of Neuropathology and Experimental Neurology
Volume42
Issue number2
DOIs
StatePublished - Mar 1983

Keywords

  • Brain neoplasms
  • CDF
  • Cellular
  • Corynebacterium parvum
  • Experimental
  • Immunity
  • Immunization
  • Nitrosourea-induced
  • Passive
  • Radiation
  • Rat glioma
  • Rats

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