Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices

M. R. Williams, R. D. Stedtfeld, H. Waseem, T. Stedtfeld, B. Upham, W. Khalife, B. Etchebarne, M. Hughes, J. M. Tiedje, S. A. Hashsham

Research output: Contribution to journalReview articlepeer-review

15 Scopus citations

Abstract

Antimicrobial resistance (AMR) is recognized as a global threat to human health. Rapid detection and characterization of AMR is a critical component of most antibiotic stewardship programs. Methods based on amplification of nucleic acids for detection of AMR are generally faster than culture-based approaches but they still require several hours to more than a day due to the need for transporting the sample to a centralized laboratory, processing of sample, and sometimes DNA purification and concentration. Nucleic acids-based point-of-care (POC) devices are capable of rapidly diagnosing antibiotic-resistant infections which may help in making timely and correct treatment decisions. However, for most POC platforms, sample processing for nucleic acids extraction and purification is also generally required prior to amplification. Direct amplification, an emerging possibility for a number of polymerases, has the potential to eliminate these steps without significantly impacting diagnostic performance. This review summarizes direct amplification methods and their implication for rapid measurement of AMR. Future research directions that may further strengthen the possibility of integrating direct amplification methods with POC devices are also summarized.

Original languageEnglish
Pages (from-to)1229-1241
Number of pages13
JournalAnalytical Methods
Volume9
Issue number8
DOIs
StatePublished - Feb 28 2017

Fingerprint

Dive into the research topics of 'Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices'. Together they form a unique fingerprint.

Cite this