Increase in Extracellular Dopamine in the Striatum During Cerebral Ischemia: A Study Utilizing Cerebral Microdialysis

Actam Slivka, Timothy S. Brannan, Jesse Weinberger, Peter J. Knott, Gerald Cohen

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105 Scopus citations

Abstract

Abstract: Unilateral ligation of the left common carotid artery in anesthetized Mongolian gerbils resulted in a steep rise in extracellular dopamine in the ipsilateral striatum in 9 out of 19 animals. Extracellular dopamine was measured by cerebral dialysis in vivo and reached a peak of 0.19 mM at 40 min. At the same time, the level of homovanillic acid fell, whereas the levels of ascorbate and 3,4‐dihydroxyphenylacetic acid remained relatively constant. In a separate group of animals studied with a combined dialysis/electrochemistry probe, a rise in the in vivo chronoamperometric signal in three out of six animals correlated with a rise in extracellular dopamine. The number of animals responding in these experiments (roughly 50%) corresponds to the frequency of incompetent Circle of Willis, as well as literature reports of the frequency of signs of stroke in unanesthetized gerbils. These results show a remarkable accumulation of dopamine in extracellular fluid in response to cerebral ischemia. Released dopamine appears to be responsible for the elevated in vivo electrochemical signal previously reported.

Original languageEnglish
Pages (from-to)1714-1718
Number of pages5
JournalJournal of Neurochemistry
Volume50
Issue number6
DOIs
StatePublished - Jun 1988

Keywords

  • Brain
  • Chronoamperometry
  • Dialysis
  • Dopamine
  • Homovanillic acid
  • Ischemia

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