Induction of ICAM-1 and HLA-DR expression by IFN-γ in malignant melanoma cell lines

J. D. Kim, J. L. Lee, J. H. Park, J. M. Lee, Y. H. Kim, S. J. Kim

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11 Scopus citations


Two human malignant melanoma cell lines, Malme-3M and SK-Mel-28, were analyzed for their ability to induce the expression of intercellular adhesion molecule 1 (ICAM-1) and human leukocyte antigen (HLA)-DR molecules on their cell surfaces as well as at the transcriptional level before and after treatment with interferon (IFN)-γ. Both cell lines demonstrated a high percentage (>99%) of ICAM-1 expression regardless of IFN-γ treatment. Before IFN-γ treatment, Malme-3M cells barely expressed HLA-DR molecules (<2%) and SK-Mel-28 cells demonstrated a relatively high percentage (>50%) of HLA-DR expression. Both cell lines displayed elevated levels of HLA-DR expression in a time dependent manner after IFN-γ treatment. However, these two cell lines have been shown to respond differentially to IFN-γ. The molecular mechanism underlying such a differential behavior was investigated, and HLA-DR gene regulation was studied at the transcriptional level. Treatment with IFN-γ led to the steady-state mRNA augmentation of the HLR-DR gene. The HLA-DRA mRNA augmentation was similar in both cell lines, whereas in Malme-3M, IFN-γ did not augment the rate of transcription of the HLA-DRB gene as much as in SK-Mel-28. Data from this study established the fact that the melanoma cell lines displayed a differential susceptibility to IFN-γ on the modulation of HLA-DR molecules, and this modulation was transcriptionally regulated.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalYonsei Medical Journal
Issue number1
StatePublished - 1995
Externally publishedYes


  • HLA-DR expression
  • ICAM-1
  • IFN-γ
  • malignant melanoma cell lines
  • transcriptional regulation


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