Inhibition of Akt2 phosphorylation abolishes the calorie restriction-induced improvement in insulin-stimulated glucose uptake by rat soleus muscle

Naveen Sharma, Edward B. Arias, Gregory D. Cartee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Calorie restriction (CR;~60%-65% of ad libitum consumption) can enhance insulin-stimulated glucose uptake (ISGU) in predominantly slow-twitch skeletal muscles (e.g., soleus) by an incompletely understood mechanism. We used an Akt inhibitor (MK-2206) to eliminate CR’s effect on insulin-stimulated Akt2 phosphorylation in isolated rat soleus muscles. We found long-term CR-enhanced ISGU was abolished by eliminating the CR effect on Akt2 phosphorylation, suggesting the CR-induced benefit on ISGU in the predominantly slow-twitch soleus relies on enhanced Akt2 phosphorylation.

Original languageEnglish
Pages (from-to)1208-1211
Number of pages4
JournalApplied Physiology, Nutrition and Metabolism
Volume41
Issue number11
DOIs
StatePublished - Jul 19 2016

Keywords

  • Dietary restriction
  • Glucose transport
  • Insulin signaling
  • Protein kinase B
  • Slow-twitch muscle

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